Upland cotton ghb2 protein and its coding gene and application
A transgenic cotton technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as loss of resistance, lack of genetic resources for upland cotton resistance to Verticillium wilt, and loss of agricultural production
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[0061] Example 1. Acquisition and functional verification of GhB2 gene in upland cotton
[0062] 1. Acquisition of GhB2 gene in upland cotton
[0063] 1. Planting of cotton materials:
[0064] Cotton (the cotton varieties are Zhongzhi cotton KV3 and 86-1 (source see above), Gossypium hirsutum L.)) seeds were delinted with sulfuric acid. Select plump cotton seeds, soak them in 70% alcohol for 5 minutes, and then use 5% H 2 o 2 Soak for 2 hours, rinse with sterile water for 3 times, then soak in sterile water for 5 hours to accelerate germination. The seeds were planted in flower pots, and the test soil was sterilized vermiculite. Culture conditions: 26 / 20°C, light for 16 hours, and darkness for 8 hours. After the cotton plant grows cotyledons, move to the medium containing 1 / 3 MS (see literature: Murashige T and Skoog F.A revised medium for rapid growth and bioassays with tobacco tissuecultures. Physiol Plant, 1962,15:473-497.) in a large beaker until the second true leaf ...
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