Preparation method of high-purity N-acetyl-D-glucosamine

Abstract

The invention provides a preparation method of high-purity N-acetyl-D-glucosamine, and relates to monosaccharide. The invention provides the preparation method of the high-purity N-acetyl-D-glucosamine with the advantages that the operation is simple, the production cost is low, and economy and environment-friendly effects are achieved. The method comprises the following steps of (1) chitin is used as a raw material, and a degradation agent of sulphuric acid is used for performing degradation reaction on the chitin; (2) filtering is performed to remove unreacted chitin, and unreacted sulphuric acid is removed by a membrane method; (3) calcium hydroxide solid is added into a solution subjected to unreacted sulphuric acid removal by the membrane method, and the pH value is regulated to be 5.5 to 7.0; (4) separated precipitates are removed through filtering, and the filtered solution is decolored by activated carbon; (5) absolute ethyl alcohol is added into the decolored solution until no precipitates separate out; (6) the precipitates are filtered and collected; the precipitates are dissolved by water; then, the absolute ethyl alcohol is added for separating out solid; next, the solid is collected and dried, and the product of N-acetyl-D-glucosamine powder is obtained. The product purity can be greater than 98 percent.

Description

technical field [0001] The invention relates to monosaccharides, in particular to a method for preparing high-purity N-acetyl-D-glucosamine. Background technique [0002] N-Acetyl-D-glucosamine (N-Acetyl-D-(+)-glucosamine, GlcNAc) is an endogenous monosaccharide, and current studies have shown that it can treat osteoarthritis, rheumatoid arthritis and anti-inflammatory Oxidation, antibacterial, antitumor and other effects. In addition, it also has a wide range of functions such as stabilizing cardiomyocytes and anti-oxidative stress. In terms of practical applications, GlcNAc has important applications in the fields of medicine, food and fine chemicals, and can be used as sweeteners for diabetics, food antioxidants and food additives for infants and young children. [0003] At present, there are two main methods for the preparation of GlcNAc. One is through the method of chemical synthesis, using (GAH) as raw material, acetic anhydride as acylating reagent, adding silver ...

AI Technical Summary

Problems solved by technology

One is through the method of chemical synthesis, using (GAH) as raw material, acetic anhydride as acylating reagent, adding silver acetate, anhydrous methanol or pyridine to react at a certain temperature, the reaction process of this method is relatively complicated, and it is easy to produce more impurities Moreover, the recrystallization conditions of the crude product are harsh, and it is difficult to ensure the yield and purity of the final product. At the same time, the use of glucosamine hydrochloride as a raw material is costly

The second is to use enzymatic hydrolysis, using chitin as raw material, and adding specific chitinase can obtain GlcNAc with higher purity, but the high price of chitinase will greatly increase the preparation cost

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