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A gene-regulated continuous fermentation hybrid power system

A power system and gene technology, applied in the direction of fermentation, chemical process analysis/design, etc., can solve the problems of continuous fermentation that have not been reported.

Inactive Publication Date: 2019-05-28
HUZHOU TEACHERS COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2013-2014, there were achievements in the identification of path parameters of the hybrid system of enzyme catalysis and gene regulation in batch fermentation, but there was no report on continuous fermentation

Method used

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  • A gene-regulated continuous fermentation hybrid power system
  • A gene-regulated continuous fermentation hybrid power system
  • A gene-regulated continuous fermentation hybrid power system

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Experimental program
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Embodiment Construction

[0050] The problem of glycerol microbial fermentation control includes control elements including biomass, extracellular glycerol, extracellular 1,3-PD, acetic acid, ethanol, intracellular glycerol, intracellular 3-HPA, and intracellular 1,3- PD, regulatory protein base sequence m R , Free regulatory protein R, glycerol dehydratase base sequence M GDHt , Glycerol dehydratase GDHt, oxidoreductase base sequence M PDOR , 1,3-PD oxidoreductase PDOR, based on actual experiments, give the following hypotheses:

[0051] 1) Considering the sequence of model building from simple to complex, DHA's activating effect on dha regulatory factors is temporarily ignored due to the complexity of the oxidation pathway, only the reduction pathway of glycerol metabolism is considered.

[0052] 2) Genes encoding related enzymes in the process of glycerol metabolism are controlled by the same promoter gene and regulatory gene.

[0053] 3) The concentration of the reactants in the reactor is consistent at ...

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Abstract

The invention relates to a gene regulation continuous fermentation hybrid power system. The system solves a glycerin microorganism fermentation control problem by utilizing an optimal scientific theory. The glycerin microorganism fermentation control problem comprises control factors; and the control factors comprise biomass, extracellular glycerin, extracellular 1,3-PD, acetic acid, ethanol, intracellular glycerin, intracellular 3-HPA, intracellular 1,3-PD, mR, free regulatory proteins R, MGDHt, glycerol dehydratase GDHt, MPDOR and 1,3-PD oxidordeuctase PDOR. According to the gene regulation continuous fermentation hybrid power system, balance is found between mathematical modeling and numerical value accuracy, a possible metabolism path is proposed, a 14-dimension nonlinear hybrid power system with discrete path parameters and continuous system parameters for continuous fermentation is established, and an optimal control problem is structured by using a constraint condition.

Description

Technical field [0001] The invention belongs to the technical field of automatic control, and specifically relates to a gene-regulated continuous fermentation hybrid power system. Background technique [0002] In the case of depletion of international petroleum resources, biodiesel is a very environmentally friendly alternative, but it will produce by-products containing about 10% glycerol during consumption, which makes glycerol production excessive, and a large amount of glycerol can be converted into very useful 1,3-Propanediol (1,3-PD). The conversion of glycerol to 1,3-PD through microbial fermentation has the advantages of easy operation, low energy consumption, and no pollution. There are several microorganisms that can convert glycerol to 1,3-PD in nature, among which Krebs Bacillus (Klebsiella pneumoniae) and Clostridium butyricum (Clostridium butyricum) have higher transformation rate and 1,3-PD production capacity, so they have received more attention. But Clostridiu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16C20/10C12P7/18
CPCC12P7/18G16C20/10
Inventor 王金鹤周丽王炜庞丽萍
Owner HUZHOU TEACHERS COLLEGE
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