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Gene regulation continuous fermentation hybrid power system

A power system and gene technology, applied in fermentation, special data processing applications, instruments, etc., can solve the problems of continuous fermentation that have not been reported

Inactive Publication Date: 2016-07-06
HUZHOU TEACHERS COLLEGE
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  • Abstract
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Problems solved by technology

[0004] In 2013-2014, there were achievements in the identification of path parameters for the hybrid system of enzyme catalysis and gene regulation in batch fermentation, but there was no report on continuous fermentation

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  • Gene regulation continuous fermentation hybrid power system
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  • Gene regulation continuous fermentation hybrid power system

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Embodiment Construction

[0046] The glycerol microbial fermentation control problem includes control elements including biomass, extracellular glycerol, extracellular 1,3-PD, acetic acid, ethanol, intracellular glycerol, intracellular 3-HPA, and intracellular 1,3-PD. PD, m R , free regulatory protein R, M GDHt , glycerol dehydratase GDHt, M PDOR, 1,3-PD oxidoreductase PDOR, according to actual experiments, the following assumptions are given:

[0047] 1) Considering the order of building the model from simple to complex, the activation of DHA on the dha regulator is not considered due to the complexity of the oxidation pathway, and only the reduction pathway of glycerol metabolism is considered.

[0048] 2) The genes encoding enzymes involved in glycerol metabolism are controlled by the same promoter gene and regulatory gene.

[0049] 3) The reactants in the reactor have the same concentration at each space point.

[0050] 4) The absolute value of the concentration difference between glycerol and ...

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Abstract

The invention relates to a gene regulation continuous fermentation hybrid power system. The system solves a glycerin microorganism fermentation control problem by utilizing an optimal scientific theory. The glycerin microorganism fermentation control problem comprises control factors; and the control factors comprise biomass, extracellular glycerin, extracellular 1,3-PD, acetic acid, ethanol, intracellular glycerin, intracellular 3-HPA, intracellular 1,3-PD, mR, free regulatory proteins R, MGDHt, glycerol dehydratase GDHt, MPDOR and 1,3-PD oxidordeuctase PDOR. According to the gene regulation continuous fermentation hybrid power system, balance is found between mathematical modeling and numerical value accuracy, a possible metabolism path is proposed, a 14-dimension nonlinear hybrid power system with discrete path parameters and continuous system parameters for continuous fermentation is established, and an optimal control problem is structured by using a constraint condition.

Description

technical field [0001] The invention belongs to the technical field of automatic control, and relates to a gene-regulated continuous fermentation hybrid power system. Background technique [0002] In the case of the depletion of international oil resources, biodiesel is a very environmentally friendly substitute, but it will produce a by-product containing about 10% glycerol during consumption, which makes glycerin excess production, a large amount of glycerol can be converted into very useful 1,3-Propanediol (1,3-PD). Converting glycerol into 1,3-PD by microbial fermentation has the advantages of easy operation, low energy consumption, and no pollution. There are several microorganisms that can convert glycerol into 1,3-PD in nature, among which Klebsiella Bacillus (Klebsiellapneumoniae) and Clostridium butyricum (Clostridium butyricum) have a higher transformation rate and 1,3-PD production capacity, and thus have received more attention. However, Clostridium butyricum r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G06F19/00C12P7/18
CPCC12P7/18G16C20/10
Inventor 王金鹤周丽王炜庞丽萍
Owner HUZHOU TEACHERS COLLEGE
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