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Method and device for detecting sample

A detection method and detection device technology, which is applied in the direction of measuring devices, particle and sedimentation analysis, instruments, etc., can solve the problems of detection efficiency reduction

Active Publication Date: 2016-07-06
艾珀尔有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

That is, the detection efficiency is lowered because the passing state of the composite particle is restricted

Method used

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  • Method and device for detecting sample
  • Method and device for detecting sample

Examples

Experimental program
Comparison scheme
Effect test

no. 1 approach

[0044] (Structure of sample detection device)

[0045] figure 1 This is a cross-sectional view showing a schematic configuration of a sample detection device used in the sample detection method according to the first embodiment.

[0046] The sample detection device is provided with a liquid-filled detection kit 1 . The detection kit 1 is divided into upper and lower parts by an insulating partition wall 2 . The first chamber 3 is formed above the partition wall 2 inside the detection kit 1 . The second chamber 4 is formed below the partition wall 2 inside the detection kit 1 . The support plate 6 is provided on the bottom surface of the partition wall 2 to support the partition wall 2 . A hole 5 in the shape of a truncated cone passes through the central portion of the support plate 6 . Minute through-holes 7 pass through the part of the partition wall 2 corresponding to the holes 5 of the support plate 6 . Therefore, the first and second chambers 3 and 4 are connected t...

no. 2 approach

[0082] In the second embodiment, two types of measurement target substances (for example, biomolecules) that may exist in a sample will be described as an example. In addition, the sample detection using the aforementioned figure 1 The sample detection device is shown.

[0083] like Figure 5A As shown, the reagent contains a first capture substance 42 (such as a first antibody) that is specifically bound to the surface of the first substance to be measured and has a size (such as a diameter) that is bound to the capture substance 42 that is smaller than that of the substance to be measured. ) of the first marker particle 43 . In addition, if Figure 5B As shown, the reagent contains a second capture substance 52 (for example, a second antibody) specifically bound to the surface of the second substance to be measured, and a second label particle 53 bound to the capture substance 52 . In addition, the sizes (for example, diameters) of the first and second labeling particles...

no. 3 approach

[0091] refer to Figure 7 and Figure 8 , and the sample detection device according to the third embodiment will be described in detail.

[0092] Figure 7 It is a plan view of the sample detection device according to the third embodiment. Figure 8 for along Figure 7 Cross-sectional view of line VIII-VIII. The eg rectangular body 61 has a rectangular block 62 . The upper groove 64 and the lower groove 65 are arranged on the rectangular block 62 with the central wall 63 as a boundary. The upper cover 66 is disposed on the upper surface of the block 62 and covers the upper groove 64 . The lower cover 67 is arranged on the bottom surface of the block 62 and covers the lower groove 65 .

[0093] A hollow first chamber 68 is formed in a space defined by the upper groove 64 of the block 62 and the upper cover 66 . A hollow second chamber 69 is formed in a space defined by the lower groove 65 of the block 62 and the lower cover 67 .

[0094] The first chamber 68 is compose...

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Abstract

According to one embodiment, a method for detecting a sample includes preparing a device for detecting a sample, the device including a measurement cassette, a first chamber formed by partitioning the cassette with a partition wall, a through-hole provided in the partition wall, a first electrode provided in the cassette, and a second electrode provided in the cassette, introducing a reagent and a sample containing a measuring object substance into the first chamber, introducing a conductive liquid into the second chamber, supplying current to the through-hole, allowing the measuring object substance whose surface is bound to and is covered by the tag particles via the capture substance in the first chamber to pass through the through-hole, and detecting presence of the measuring object substance.

Description

[0001] This application is based on Japanese patent application 2014-263899 (filing date: December 26, 2014) and Japanese patent application 2015-221337 (filing date: November 11, 2015), and enjoys the priority rights of these applications. This application incorporates these applications in their entirety by reference. technical field [0002] Embodiments relate to a sample detection method and a sample detection device for detecting viruses, bacteria, and the like in a sample. Background technique [0003] In recent years, infectious diseases caused by viruses, such as Ebola hemorrhagic fever and dengue fever, have rapidly spread around the world and are becoming social problems. Proper isolation and other treatment of patients after the onset of illness is very important to prevent panic. Furthermore, in the future, it will also be important to accurately separate patients who are sick but not sick, and prevent the spread of pathogenic viruses from patients before they h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569
CPCG01N33/56911G01N33/56983G01N2333/08G01N2333/185G01N33/54346G01N33/54366G01N15/12B82Y5/00B82Y15/00G01N33/48728G01N2015/133G01N27/40
Inventor 本乡祯人宫本浩久
Owner 艾珀尔有限公司
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