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Molecular markers of major QTL for rice heading period and application of molecular marker

A molecular marker and heading stage technology, which is applied in the field of molecular genetics and breeding, can solve the problems of limiting the application of rice heading stage and the number of heading stages, etc., and achieves the effect of saving time and reducing labor costs.

Active Publication Date: 2016-07-06
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of cloned heading date is too small, which greatly limits the application of rice heading date in production practice. Therefore, it is urgent to strengthen the isolation of rice heading date quantitative trait loci

Method used

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  • Molecular markers of major QTL for rice heading period and application of molecular marker
  • Molecular markers of major QTL for rice heading period and application of molecular marker
  • Molecular markers of major QTL for rice heading period and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Obtaining of Molecular Markers Closely Linked to Heading Stage Main Effect Genes

[0021] (1) Positioning groups and test methods

[0022] 1. Positioning groups

[0023] Zhan Xiaodeng and others initially positioned qHD5 between molecular markers RM17788 and RM5374. On this basis, the present invention further expands the positioning group, adopting the BC 4 f 2 BC 4 f 3 High generation segregation groups.

[0024] The present invention utilizes BC 4 f 3 The segregating populations were first genetically analyzed for qHD5, as figure 2 As shown, the segregation ratio of early heading and late heading is consistent with 1:3 (χ 2 =1.0159, P=0.3135>0.05), indicating that the gene is controlled by a single Mendelian factor, and late heading is dominant over early heading. figure 1 Shown is a comparison of rice phenotypes containing DTH5 and dth5.

[0025] The present invention utilizes BC 4 f 3 More than 2000 extreme recessive individuals in the high-...

Embodiment 2

[0036] Embodiment 2 (Utilize closely linked molecular marker H70 and 301K to identify, select the method for the suitable heading date variety of rice)

[0037] Specific steps are as follows:

[0038] (1) Extraction of DNA: Get the young leaves of rice, and extract genomic DNA by CTAB method;

[0039] (2) Establishment of a standard PCR amplification system: with any one or two pairs of labeled primers in H70 and 301K, the whole genome DNA of a single rice plant to be tested is used as a template to perform PCR amplification;

[0040] (3) Detection and analysis of amplified DNA fragments: PCR products are detected by 8% polyacrylamide gel electrophoresis. If the marker H70 can amplify a 171bp fragment, it indicates that the single plant genotype is DTH5, and its phenotype is late heading If a 165bp fragment can be amplified, it indicates that the individual plant genotype is dth5, and its phenotype is early heading; if a heterozygous band type can be amplified, it indicates t...

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Abstract

The invention discloses molecular markers of major QTL for the rice heading period and application of the molecular marker.The molecular markers include an H70 primer pair and a 301K primer pair, wherein the H70 primer pair is composed of a sequence shown as SEQ ID No.1 and a sequence shown as SEQ ID No.2; the 301K primer pair is composed of a sequence shown as SEQ ID No.3 and a sequence shown as SEQ ID No.4; the two molecular markers are closely linked with QTL for the heading period and can be used for authenticating and breeding varieties for the rice heading period.The invention further discloses a method for authenticating and breeding the varieties for the rice heading period through the molecular markers.The major QTL which are located on the galianconism of the fifth chromosome and used for controlling the heading period are precisely positioned for the first time, and the InDel markers H70 and 301K which are closely linked are obtained.Whether variation of the major QTL for the heading period exists or not can be judged and authenticated just by detecting the characteristics of amplified bands of the markers, the phenotype of rice in the heading period can be predicted, and the molecular markers are used for guiding breeding work for an improvement in the rice heading period.

Description

technical field [0001] The invention belongs to the field of molecular genetic breeding, in particular, it relates to a molecular marker of a major QTL for heading stage in rice and an application thereof. Background technique [0002] Rice is one of the most important food crops in the world. Rice is grown in more than 110 countries and regions around the world. Nearly half of the population in my country eats rice as a staple food. Rice production plays an important role in ensuring food security in my country. However, with the development of modern agricultural production and society, intensive and modern cultivation techniques have put forward higher requirements for the cultivation characteristics of rice, especially regional adaptability. Traditional rice varieties can no longer meet the production needs. High-quality, wide-adapted new varieties have become the common goal of rice breeders. Heading date is one of the important agronomic traits of rice, and the length...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 占小登孙滨程式华曹立勇张迎信沈希宏
Owner CHINA NAT RICE RES INST
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