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A method for observing and processing aphid embryos

A treatment method and aphid technology, applied in the direction of instruments, analytical materials, measuring devices, etc., can solve problems such as salinity, rupture of ovarian tubes, incompleteness, etc., and achieve the effect of overcoming defects

Inactive Publication Date: 2019-03-26
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defects of the prior art, avoid the rupture and incompleteness of the ovarian tube caused by the embryo imbibition caused by the osmotic action of the solution, and the endogenous lysosomal enzymes of the embryo tissue to the self tissue and Blurred vision and unclear imaging defects caused by cell autolysis, as well as salt stains caused by solution solvent evaporation; the invention provides a simple and effective method for embryo tissue processing, through which observation and counting can be simple and effective , while facilitating imaging

Method used

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  • A method for observing and processing aphid embryos
  • A method for observing and processing aphid embryos
  • A method for observing and processing aphid embryos

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Under normal temperature and pressure, use a pipette to take 20ml of absolute ethanol, 1ml of ethyl acetate, and 1ml of glycerin; store in a solution bottle and seal it for later use.

[0029] Use a dropper to drop a drop of distilled water on the concave slide, and move a wingless or winged aphid of different ages into the water of the concave slide with a fine brush to prevent the aphids from escaping; set aside.

[0030] Under the binocular stereo dissecting microscope, use two 0-size insect needles to hold the ventral surface of the aphid upward, one insect needle is fixed on the mid-thoracic plate of the aphid, and the other insect needle is gently pressed from front to back along the center of the abdomen. Gently cut open, press the reproductive abdomen with an insect needle to pull out all the ovaries, and remove the mother's shell and other sundries.

[0031] Add 2 drops of the prepared observation solution to completely immerse the embryonic tissue, and the out...

Embodiment 2

[0034] Under normal temperature and pressure, use a pipette to take 30ml of absolute ethanol, 1ml of ethyl acetate, and 1ml of glycerin; store in a solution bottle and seal it for later use.

[0035] Use a dropper to drop a drop of distilled water on the concave slide, and move a wingless or winged aphid of different ages into the water of the concave slide with a fine brush to prevent the aphids from escaping; set aside.

[0036] Under the binocular stereo dissecting microscope, use two 0-size insect needles to hold the ventral surface of the aphid upward, one insect needle is fixed on the mid-thoracic plate of the aphid, and the other insect needle is gently pressed from front to back along the center of the abdomen. Gently cut open, press the reproductive abdomen with an insect needle to pull out all the ovaries, and remove the mother's shell and other sundries.

[0037] Add 2 drops of the prepared observation solution to completely immerse the embryonic tissue, and the out...

Embodiment 3

[0040] Under normal temperature and pressure, use a pipette to take 50ml of absolute ethanol, 1ml of ethyl acetate, and 1ml of glycerin; store in a solution bottle and seal it for later use.

[0041] Use a dropper to drop a drop of distilled water on the concave slide, and move a wingless or winged aphid of different ages into the water of the concave slide with a fine brush to prevent the aphids from escaping; set aside.

[0042] Under the binocular stereo dissecting microscope, use two 0-size insect needles to hold the ventral surface of the aphid upward, one insect needle is fixed on the mid-thoracic plate of the aphid, and the other insect needle is gently pressed from front to back along the center of the abdomen. Gently cut open, press the reproductive abdomen with an insect needle to pull out all the ovaries, and remove the mother's shell and other sundries.

[0043] Add 2 drops of the prepared observation solution to completely immerse the embryonic tissue, and the out...

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Abstract

The invention discloses a method for observing and processing aphid embryos, which uses a newly prepared observation liquid. The observation liquid can enhance the toughness of the ovarian tube wall membrane tissue and make the embryos less likely to scatter. It can also precipitate proteins in the embryos and cells to enhance the visual effect, thereby preventing This method solves the countless and unclear problems caused by the easy scattering of embryos in the ovarian tubes and the autolysis of cell lysosomes in the use of the original technology, laying the foundation for future research on the differences in aphids' reproductive characteristics. This method is safe, simple, fast, and has obvious effects; for researchers or research units that lack special imaging system equipment during research and observation, this method can be used to complete the number of aphid ovarian tubes and each Counting the number of embryos in the ovarian tubes.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to a method for observing and treating aphid embryos. Background technique [0002] Aphids are a typical group of model insects to study ecology, development and evolution. Especially after the International Aphid Genome Consortium sequenced the whole genome of the pea aphid in 2010, it provided additional information for further research on evolutionary biology and developmental biology. Biologically incomparable conditions. Aphid polymorphism is very common, not only with color type and wing type differentiation, but also with reproductive polymorphism. Parthenogenesis and sexual reproduction are two modes of reproduction in aphids. The 5,000 known species of aphids in the world so far use parthenogenesis as the main reproductive mode in their annual life cycle. Due to the fast reproduction speed of parthenogenesis, the short generation cycle and the large numbe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/10G01N1/28G01N21/84
CPCG01N1/2813G01N15/10G01N21/84G01N2015/1024
Inventor 张廷伟刘长仲王国利
Owner GANSU AGRI UNIV
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