Molecular marker closely interlocked with neck blast resistance gene of paddy rice Jiangnan lateness and application thereof

Abstract

The invention belongs to the field of crop molecular genetic thremmatology, and discloses a molecular marker interlocked with a neck blast resistance gene of paddy rice Jiangnan lateness and an application thereof. The molecular marker is prepared by the following steps: (1) acquiring a paddy rice sample, and extracting a genome DNA of the paddy rice sample; (2) performing PCR amplification for the genome DNA of the paddy rice sample by utilizing a primer pair of any pair of molecular markers in RM27273 and BS33, and performing the electrophoresis detection on a PCR amplification product, wherein if a molecular marker DNA fragment of the corresponding size is amplified, the existence of the Pb-jn1 gene is marked. By virtue of the molecular marker of the paddy rice neck blast resistance gene Pb-jn1, whether the paddy rice Jiangnan lateness as well as the hybrid, the backcross and the multiple-cross later generations of the paddy rice Jiangnan lateness contain the gene can be detected, the resistance level to the paddy neck blast can be predicted, the selection efficiency of the paddy rice neck blast resistance material can be greatly improved, and the anti-disease breeding process can be accelerated.

Description

technical field [0001] The invention belongs to the field of crop molecular genetics and breeding, and relates to a molecular marker closely linked with rice Jiangnan late panicle blast resistance gene and its application. Background technique [0002] Rice is one of the most important food crops in our country, which is of great significance to ensure our country's food security and national economic growth. Rice blast is the most serious disease in rice production in my country, and has the characteristics of rapid spread, wide occurrence, and severe damage (Ling Zhongzhuan et al., 1989, Research on the Physiological Race of Magnaporthe grisea in Northern my country. Agricultural Sciences of China, 22(3 ):7-13). It is the most economical and effective way to control the occurrence of rice blast and reduce the loss of rice yield by further exploring and utilizing the genetic resources of rice blast resistance in my country and cultivating and planting disease-resistant vari...

AI Technical Summary

Problems solved by technology

However, traditional breeding methods are time-consuming, laborious, phenotypic identification is difficult, and breeding efficiency is low. Since most disease resistance genes are dominant and epistasis interactions often exist between genes, the aggregation of disease resistance genes is even more difficult.

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