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Measuring system for obtaining single molecular spectrum and imaging under shear field

A molecular spectroscopy and imaging measurement technology, applied in the basic research field of polymer physics, which can solve the problems that cannot be studied, is not suitable for multi-charge system structure measurement, and cannot meet the needs of multi-charge system research, and achieves high dynamic shear measurement. , the effect of high sensitivity

Inactive Publication Date: 2016-06-22
INST OF CHEM CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the multi-charge, long-range electrostatic interaction and the existence of a large number of counter ions in this kind of soft matter system, the rheological properties of the system are very special, and many problems have not been understood by people.
[0003] However, none of these methods can study the relationship between the microstructure information of the studied system and its macroscopic rheological properties from the perspective of a single molecule, especially the light scattering technique is limited by the solution concentration and the scattering cross section, and is not suitable for the structure measurement of the multi-charge system. Therefore, it cannot meet the needs of multi-charge system research.

Method used

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  • Measuring system for obtaining single molecular spectrum and imaging under shear field
  • Measuring system for obtaining single molecular spectrum and imaging under shear field
  • Measuring system for obtaining single molecular spectrum and imaging under shear field

Examples

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Effect test

Embodiment 1

[0029] Example 1: Acquiring the single-molecule fluorescence spectrum and fluorescence correlation spectrum under the shear field, the specific process is:

[0030] 1. Place the present invention on an optical platform, move the rheometer directly above the microscope objective lens 31 through a high-precision translation stage, adjust each optical device so as to meet the optical path transmission conditions of the present invention,

[0031] 2. Place the sample to be tested on the center of the glass slide in the rheometer, and the glass slide is a quartz glass slide with a thickness of 0.13-0.17mm;

[0032] 3. Turn on the laser of 1 in the excitation light source unit, so that the laser emitted by the laser is expanded in two stages by the beam expander, and the expanded laser is collimated to become parallel light;

[0033] 4. Switch the microscope objective lens 31 to a water immersion objective lens, add 20-100 μL of ultrapure water to the microscope objective lens 31, a...

Embodiment 2

[0038] Example 2: Complete the measurement of single-molecule fluorescence imaging under the shear field, the specific process is:

[0039] 1. Place the present invention on an optical platform, move the rheometer directly above the microscope objective lens 31 through a high-precision translation stage, adjust each optical device so as to meet the optical path transmission conditions of the present invention,

[0040] 2. Place the sample to be tested on the center of the glass slide in the rheometer, and the glass slide is a quartz glass slide with a thickness of 0.13-0.17mm;

[0041] 3. Turn on the laser of the excitation light source unit 1, so that the laser light emitted by the laser 10 is adjusted by the beam expander to focus the excitation light at the negative focal plane of the microscope objective lens 31, so that it is emitted as parallel light after passing through the microscope objective lens 31;

[0042] 4. Switch the microscope objective lens 31 to an oil imme...

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Abstract

The invention relates to a measurement system for obtaining a single molecular spectrum and imaging under a shear field, characterized in that the measurement system includes: an excitation light source unit used as excitation light to irradiate a sample to be tested; a unit for applying a mechanical A shear application and rheological measurement unit for simultaneously measuring its macroscopic rheological properties; and an optical microscopic unit, the optical microscopic unit is used to introduce the excitation light emitted by the excitation light source unit into the waiting area under the shear field. The sample is measured, so that the dye molecules of the sample to be tested are excited to generate fluorescent signals, and the generated fluorescent signals are collected and respectively transmitted to a single-molecule fluorescence imaging unit, a single-molecule fluorescence emission spectrum measurement unit and / or a fluorescence correlation spectrum measurement unit . The invention can be widely applied to polyelectrolyte dilute solution, gel, interfacial diffusion, film imaging and other multi-charge condensed state systems, and explores the basic physical origin of its special properties.

Description

technical field [0001] The invention belongs to the field of fundamental research of macromolecular physics, in particular to a single molecular spectrum acquisition and imaging measurement system under a shear field. Background technique [0002] The physical origin of the rheological properties of multi-charged soft matter systems has always been an important basic research work in this field. Research in this area will not only help us design new functional materials, such as: water treatment substances, smart drugs, tissue engineering materials, etc., but also help us understand basic life processes. However, due to the multi-charge, long-range electrostatic interaction and the existence of a large number of counter ions, the rheological properties of this kind of soft matter system are very special, and many problems have not been understood. Instruments that can simultaneously measure the macroscopic rheological properties of soft matter systems such as polymers and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6458G01N2021/6419
Inventor 郑锴锴杨京法任伟斌赵江
Owner INST OF CHEM CHINESE ACAD OF SCI
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