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Molecular identification method and identification primer for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb.

A technology of Vietnamese huanghuali and Hainan huanghuali, applied in the field of molecular identification methods and identification primers of Hainan huanghuali and Vietnamese huanghuali, can solve the problems of inaccurate identification and distinction of Hainan huanghuali and Vietnamese huanghuali, and achieve strong application value Effect

Active Publication Date: 2016-06-22
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a molecular identification method and identification of Hainan huanghuali and Vietnamese huanghuali based on the sequence difference of plastid gene rpoC2 and petD in view of the deficiency that traditional wood identification methods cannot accurately identify and distinguish Hainan huanghuali and Vietnamese huanghuali Primer

Method used

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  • Molecular identification method and identification primer for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb.
  • Molecular identification method and identification primer for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb.
  • Molecular identification method and identification primer for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Determination of rpoC sequence and petD sequence of standard samples of Hainan Huanghuali and Vietnamese Huanghuali

[0050] In the early stage of the present invention, 5 Hainan huanghuali samples with flowers or fruits and capable of botanical identification were collected in the scientific research area of ​​the South China Botanical Garden and in multiple different locations in Hainan, and 5 samples with flowers or fruits were collected in multiple different locations in Hanoi, Vietnam. fruit, Vietnamese huanghuali samples that can be botanically identified. For the samples collected and identified above, the total DNA was extracted and purified according to the method described to obtain the total DNA of each sample that can be used for PCR amplification. Select two plastid DNA fragments: rpoC2 and petD as target genes. Forward / reverse primers rpoC2-Tu1F / rpoC2-Tu1R designed to amplify the plastid gene rpoC2, the sequences of which are: 5'-GAAGGTCATGCAGG...

Embodiment 2

[0055] Example 2: Identification of unknown samples 1

[0056] A wood sample was purchased at Guangzhou Yuzhu Timber Market. The sample number is TuTY1258. The merchant said the sample was Hainan Huanghuali. Through measurement, the wood color of the sample was dark yellow, and the air-dry density was 0.89g / cm 3 , the wood anatomy observation showed that the wood sample had the wood characteristics of Cedarwood, but the wood anatomy characteristics could not distinguish whether the sample was Hainan huanghuali or Vietnamese huanghuali. Therefore, we used the method of comparing the sequences of the plastid genes rpoC2 and petD for identification. The specific implementation steps are as follows:

[0057] First extract and purify the total DNA of the unknown wood sample according to the above method, and then use rpoC2 forward / reverse primers rpoC2-Tu1F / rpoC2-Tu1R: 5'-GAAGGTCATGCAGGTGGAGT-3' / 5'-CCAAAGTCGTGTGGGAAAAAGT-3' and petD Forward / reverse primers petD-Tu1F / petD-Tu1R: 5'...

Embodiment 3

[0058] Example 3: Identification of unknown samples 2

[0059] A wood sample was purchased in Fujian, the sample number is TuTY1259, and the merchant called the sample Hainan Huanghuali. Through measurement, the material color of this sample is light yellow, and the air-dry density is 0.92g / cm 3 , the wood anatomy observation showed that the wood sample had the wood characteristics of Cedarwood, but the wood anatomy characteristics could not distinguish whether the sample was Hainan huanghuali or Vietnamese huanghuali. Therefore, we used the method of comparing the sequences of the plastid genes rpoC2 and petD for identification. The specific implementation steps are as follows:

[0060] First extract and purify the total DNA of the unknown wood sample according to the above method, and then use rpoC2 forward / reverse primers rpoC2-Tu1F / rpoC2-Tu1R: 5'-GAAGGTCATGCAGGTGGAGT-3' / 5'-CCAAAGTCGTGTGGGAAAAAGT-3' and petD Forward / reverse primers petD-Tu1F / petD-Tu1R: 5'-TCCTTTGTATGTAAAA...

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Abstract

The invention discloses a molecular identification method and identification primer for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb. This method is a molecular identification method provided for differences between mesenchymal genes rpoC2 and petD sequences of Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb. The method comprises: subjecting a sample to be identified to total DNA extraction, PCR (polymerase chain reaction) amplification of rpoC2 and petD sequences, sequencing of the sequences, and comparison with rpoC2 and petD sequences of standard samples of Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb. The method enables quick and accurate identification of Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb., solves the problem that it is difficult to identify Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb. by using traditional wood dissecting means, an efficient and reliable identification method for Dalbergia odorifera T. Chen and Dalbergia rimosa Roxb. is provided for wood market, merchants, consumers, public security department, customs and scientific research institutes, and the method is highly worthy of application.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to a molecular identification method and identification primers for Hainan huanghuali and Vietnamese huanghuali. Background technique [0002] Hainan huanghuali, also known as Dalbergia ordofera T.Chen, is a plant of the genus Dalbergia L. in the leguminous family. The sales price of huanghuali wood has exceeded 10,000 yuan. The huge profit driven by the high market price of Hainan huanghuali has led to a large number of illegal acts of smuggling and selling counterfeit goods in the market. Through visiting the mahogany market and merchants, we found that more than 90% of the Hainan huanghuali currently on the market are produced in Vietnam, and the Vietnamese huanghuali (also known as Vietnamese Dalbergia tonkinensis Prain), which is less than half the price of Hainan huanghuali, is smuggled into my country and pretends to be Hainan. Huanghua pear. The reaso...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895
Inventor 涂铁要李世晋李永泉朱成杰李显强刘俊芳张奠湘
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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