Preparation method of monoamine oxidase inhibitor

A technology for monoamine oxidase and inhibitors, which is applied in the field of preparation of monoamine oxidase inhibitors, can solve the problems of few types and obvious toxic and side effects of chemically synthesized preparations, and achieve good safety, easy popularization and application, and strong inhibitory effect

Active Publication Date: 2016-06-22
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for preparing monoamine oxidase inhibitors in view of the fact that there are few types of monoamine oxidase inhibitors and the problem of obvious toxic and side effects of chemically synthesized preparations

Method used

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  • Preparation method of monoamine oxidase inhibitor
  • Preparation method of monoamine oxidase inhibitor
  • Preparation method of monoamine oxidase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Separation and purification of monoamine oxidase inhibitors from the MRS fermentation broth of Lactobacillus casei BDII

[0057] (1) prepare the fermented liquid of Lactobacillus casei BD-II:

[0058] Lactobacillus casei BD-II (CGMCC No.0849) frozen at -80°C was inoculated on the MRS plate and cultured anaerobically at 37°C for 48 hours; a single colony was picked from the plate and inoculated in 10 mL of MRS liquid medium at 37 The primary seed liquid of BD-II was obtained by anaerobic culture at ℃ for 12 hours; then the primary seed liquid was inoculated into 200mL MRS liquid medium with a 4% inoculum size for expanded culture, and the similar step-by-step expansion culture was carried out to 6L. In BIOTRONLiFlusSP_200L In a fully automatic fermenter at (35.5±0.5)°C, inoculate 6L of seed solution in 120LMRS medium for 24 hours according to 5% inoculum size, and ferment for 24 hours to obtain 126L of fermentation stock solution (pH up to 4.00, the number of c...

Embodiment 2

[0080] Example 2 Separation and purification of monoamine oxidase inhibitors from the skim milk fermentation broth of Lactobacillus casei ATCC334

[0081] (1) prepare the fermented liquid of Lactobacillus casei ATCC334:

[0082] Lactobacillus casei ATCC334 frozen at -80°C was inoculated on the MRS plate and cultured anaerobically at 37°C for 48 hours; a single colony was picked from the plate and inoculated in 5 mL of skim milk and cultured anaerobically at 37°C for 12 hours to obtain a strain of ATCC334 Grade seed liquid; then inoculate this grade 1 seed liquid into 200mL skim milk with 4% inoculum size to carry out expansion culture, similar step by step expansion culture to 6L, in BIOTRONLiFlusSP_200L automatic fermenter at (30.0 ± 0.5) ℃ Conditions: Inoculate 6L of seed liquid into 120L of skim milk and ferment for 48 hours according to 5% inoculum size, and obtain 126L of fermentation stock solution (pH reaches 4.53, and the number of colonies reaches 10 9 ).

[0083] (...

Embodiment 3

[0097] Example 3 Separation and purification of monoamine oxidase inhibitors from the skim milk fermentation broth of Lactobacillus bulgaricus LB340

[0098] (1) prepare the fermented liquid of Lactobacillus bulgaricus LB340:

[0099] Lactobacillus bulgaricus LB340 [DuPont Danisco (China) Co., Ltd.] frozen at -80°C was inoculated on the MRS plate and cultured anaerobically at 37°C for 48 hours; a single colony was picked from the plate and inoculated in 10 mL of skim milk in The primary seed liquid of LB340 was obtained by anaerobic culture at 37°C for 12 hours; then the primary seed liquid was inoculated into 200mL skim milk with a 4% inoculum size for expanded culture, and the similar step-by-step expansion culture was carried out to 6L, and the BIOTRONLiFlusSP_200L automatic In a fermenter at (40.0±0.5)°C, inoculate 6L of seed liquid into 120L of skim milk and ferment for 24 hours according to the inoculum size of 5%, to obtain 126L of fermentation stock solution (pH up to ...

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Abstract

The invention discloses a preparation method of a monoamine oxidase inhibitor. The preparation method comprises following steps: (1) ethyl alcohol is used for precipitating a lactobacillus fermentation broth, and a supernatant is collected; (2) water and ethyl acetate are added into the supernatant for extraction, an obtained ethyl acetate phase extracting solution is subjected to evaporation so as to remove the solvent and obtain an ester phase extract; and (3) the ester phase extract obtained in step (2) is subjected to column chromatography positive phase elution. The monoamine oxidase inhibitor is prepared from natural products, is obtained via separation purification from lactobacillus fermentation products, is low in toxicity, high in safety, and possesses a promising application prospect.

Description

technical field [0001] The invention relates to the fields of food, medicine and health products, in particular to a preparation method of a monoamine oxidase inhibitor. Background technique [0002] Population aging is a major problem facing mankind in the 21st century. Aging-related diseases, such as Alzheimer's disease (senile dementia), Parkinson's syndrome, etc., have gradually become the enemy of human health and a heavy burden on social prosperity, and effective drug treatment is essential to alleviate the disease . The root causes of such diseases are mostly related to the abnormal increase of monoamine oxidase activity in the human body. Therefore, finding or synthesizing substances with targeted inhibition of monoamine oxidase activity has become one of the important directions for the development of drugs for such diseases. [0003] Monoamine oxidase (monoamineoxidase, MAO, EC1.4.3.4), the full name is monoamine oxidoreductase, is a kind of flavoproteinase, main...

Claims

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Application Information

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IPC IPC(8): C07C69/68C07C67/48C12P7/62A61P25/00A61P25/28A61P25/24C12R1/245C12R1/225C12R1/23
Inventor 游春苹吴正钧郭本恒刘振民韩瑨黄奇望
Owner BRIGHT DAIRY & FOOD
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