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Efficient gamete/embryo vitrification thawing method

A vitrification and gamete technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of large cross-section of the carrier rod, liquid nitrogen tank and liquid nitrogen waste, etc., to improve efficiency and improve efficiency , cost-saving effect

Active Publication Date: 2016-06-15
LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The problem when using the above-mentioned carrier device is: because the temperature of liquid nitrogen is -196 degrees, the blade for storing the embryo needs to be installed on a longer handle, and because the blade is soft, a sleeve must be added outside the blade, which will cause The cross-section is larger and longer, and the embryo freezing rod is about 21 cm long
This results in a great waste of liquid nitrogen tanks and liquid nitrogen

Method used

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  • Efficient gamete/embryo vitrification thawing method
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  • Efficient gamete/embryo vitrification thawing method

Examples

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Embodiment 1

[0033] Embodiment 1: A kind of high-efficiency gamete / embryo vitrification freezing and thawing method, the freezing device (such as Figure 1-Figure 4 shown) includes a cryocarrier 1 and a spoon-shaped body 2 for loading gametes or embryos, the inner surface of the spoon-shaped body is provided with an anti-detachment layer, and the cryocarrier is provided with 7 through holes 11, the said A corresponding numeral 3 is provided on the carrier next to the through hole. The spoon-shaped bodies are placed in the through holes of the carrier and fixed by the fixing component, and the number of the spoon-shaped bodies is the same as the number of the through holes of the carrier. A handle 21 is also connected to the spoon-shaped body. The fixed component is a fixed rod 4 located near the through hole 11 of the carrier. The bottom surface of the handle of the spoon-shaped body is provided with a groove that matches the fixed rod. The spoon-shaped body is placed in the through hole ...

Embodiment 2

[0039] Embodiment 2: A kind of high-efficiency gamete / embryo vitrification freezing and thawing method, the freezing device (such as Figure 5-Figure 7 (shown) includes a freezing carrier 1 and a spoon-shaped body 2 for loading gametes or embryos, the inner surface of the spoon-shaped body is provided with an anti-detachment layer, and seven through holes 11 are opened on the said freezing carrier. Placed in the through hole of the carrier and fixed by the fixing component, the number of scoops is the same as that of the through hole of the carrier. The fixing assembly includes tooth I 111 arranged on the edge of the through hole and tooth II 22 arranged on the bottom surface of the spoon-shaped body, the spoon-shaped body is placed in the through hole of the carrier, and the tooth II on the bottom surface of the spoon-shaped body is located in the tooth I on the edge of the through hole And fit it tightly. The specific operation steps of this embodiment are the same as those...

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Abstract

The invention relates to an efficient gamete / embryo vitrification thawing method.A freezing device used in the method comprises a freezing carrier and spoon-shaped bodies used for containing gametes or embryos.Multiple through holes are formed in the freezing carrier.The spoon bodies are placed in the through holes in the carrier and fixed through fixing assemblies.The number of the spoon-shaped bodies is the same as that of the through holes in the carrier.The method comprises the following steps that A, the gametes or embryos which are protected in a freezing mode are placed to the bottoms of the spoon-shaped bodies under a microscope and then rapidly placed into liquid nitrogen; B, in the liquid nitrogen environment, the spoon-shaped bodies are placed in the through holes of the freezing carriers and fixed through the fixing assemblies; C, the freezing carrier is placed in a support and then placed in a liquid nitrogen tank; D, thawing is performed.The freezing efficiency can be improved, and the cost spent for buying the liquid nitrogen tank and liquid nitrogen is greatly saved.In addition, the spoon-shaped bodies are thick, freezing operation is performed under the microscope, and therefore the phenomenon that the embryos or gametes and oocytes are lost is avoided.

Description

technical field [0001] The invention relates to a high-efficiency gamete / embryo vitrification freezing and thawing method. Background technique [0002] The vitrification loading rod used in the existing method is a frozen blade with a flat front end made of a polymer material. During use, oocytes or embryos are placed on the blade for cryopreservation, and it is also convenient for future needs. It is time to thaw and recover, but the carrier device is completed under the supervision of normal eyesight. The leaves with oocytes or embryos are quickly frozen with liquid nitrogen, and plastic tubes are placed on the outside, and then put into the Store in freezing liquid. [0003] The problem when using the above-mentioned carrier device is: because the temperature of liquid nitrogen is -196 degrees, the blade for storing the embryo needs to be installed on a longer handle, and because the blade is soft, a sleeve must be added outside the blade, which will cause The cross-se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0284
Inventor 李楠李忻琳韦立红唐永梅牟联俊王丹冯晶罗婷
Owner LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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