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Penicillium janthinellum for decomposing hard-soluble aluminum phosphate in acid soil and application thereof

A technology of Penicillium purpuricum and acidic soil, which is applied in soil remediation and biological fields to achieve the effects of increasing content, reducing soil erosion and increasing yield

Inactive Publication Date: 2016-06-08
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a species of Penicillium, there are few reports on its ability to solubilize phosphorus, especially insoluble phosphorus in acidic soils.

Method used

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  • Penicillium janthinellum for decomposing hard-soluble aluminum phosphate in acid soil and application thereof
  • Penicillium janthinellum for decomposing hard-soluble aluminum phosphate in acid soil and application thereof
  • Penicillium janthinellum for decomposing hard-soluble aluminum phosphate in acid soil and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preliminary screening of embodiment 1 phosphorus-solubilizing microorganism

[0019] Weigh 10g of water-soaked feces and biogas residue provided by the pig farm of Wuhan Tianjian Agricultural Development Co., Ltd. into a triangular flask filled with 90mL of sterile saline, and shake at a constant temperature of 180r / min on a constant temperature shaker at 28°C for 30min to disperse the sample evenly In distilled water, stand for 20 minutes and take the supernatant to obtain a dilution factor of 10 -1 Bacterial suspension, diluted sequentially to obtain 10 -3 、10 -5 、10 -7 Bacterial suspension of double dilution, respectively take 0.1ml of bacterial suspension of each concentration gradient and spread evenly on the solid medium containing aluminum phosphate (AlPO 4 , 5g / L), each concentration was repeated three times, placed in a constant temperature incubator, and incubated at a constant temperature of 30°C for 5-7 days. Observe the colonies grown on the medium,...

Embodiment 2

[0020] The re-screening of embodiment 2 phosphorus-solubilizing microorganisms

[0021] The strains obtained from the primary screening were inoculated into 100 mL of inorganic phosphorus liquid medium containing aluminum phosphate (AlPO 4 , 5g / L), 30°C, 180rpm, cultivated for 15 days, and at the same time, the liquid phosphate-dissolving medium without adding any bacteria was used as a control group. After the culture solution was centrifuged at 5000r / min for 10min, the supernatant was taken, diluted to an appropriate concentration, and the content of soluble phosphorus was determined by the molybdenum-antimony anti-colorimetric method. The result is as figure 1 Shown, above-mentioned 6 bacterial strains screened by the present invention all have certain phosphorus-dissolving effect, can remove insoluble phosphorus (AlPO) in the culture medium 4 ) into soluble phosphorus, among which the PA01 strain had the highest phosphorus-dissolving efficiency, and the dissolved phospho...

Embodiment 3

[0022] Identification of embodiment 3 bacterial strain PA01

[0023] (1) Colony morphology of strain PA01

[0024] The colonies are white, velvety in texture, with round and neat edges; the colonies are colorless on the back or slightly yellow in the center. There are a large number of conidia on the front of the colony, with light yellow exudate; the flora is white, the hyphae are developed, the conidiophores are tubular, and conidia are produced on them, and the conidia are round.

[0025] (2) Molecular biological identification of strain PA01

[0026] The PA01 strain obtained from the above screening was identified by molecular biology methods, and the 18SrDNAITS sequence of the strain was amplified by PCR to obtain an amplified product with a length of about 600 bp. The amplified product was sequenced by a sequencing company, and the measured sequence was compared with The sequence in the GenBank database was compared by BLAST, and the result showed that the similarity b...

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Abstract

The invention discloses penicillium janthinellum PA01 for decomposing hard-soluble aluminum phosphate in acid soil. The penicillium janthinellum PA01 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO:M2015757. According to the strain, wild phosphate solubilizing bacterium genetic resources are enriched, a reserve library for phosphate solubilizing bacterium whole-genome breeding is expanded, the content of available phosphorus in the acid soil can be increased, the utilization efficiency of phosphate fertilizer can be improved, the yield of crops can be increased, water and soil loss can be reduced, and wide application prospects are achieved.

Description

technical field [0001] The invention belongs to the field of soil remediation and biotechnology, in particular to a strain of Penicillium janthinellum PA01 and its dissolving effect on insoluble aluminum phosphate, which can be applied to dissolve insoluble phosphorus in acidic soil. technical background [0002] Phosphorus plays an important role in plant growth and development, but most of the phosphorus in soil cannot be directly absorbed by plants. According to the survey, 74% of the cultivated land in the country is deficient in phosphorus. In order to meet the production needs, most of the farmland in my country needs to apply a large amount of phosphorus fertilizer, but the seasonal utilization rate of the applied phosphorus fertilizer is only 5%-25%. Most of the phosphorus and soil Ca in 2+ , Fe 3+ 、Al 3+ etc. combined to form insoluble phosphate, which cannot be absorbed and utilized by plants. In acidic soil, it mainly exists in the form of Al-P and Fe-P. Theref...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/80C09K109/00
CPCC09K17/14C09K2109/00C12N1/145C12R2001/80
Inventor 朱端卫李震宇肖乃东周洋肖雪周文兵蔡建波赵建伟万小琼刘广龙华玉妹
Owner HUAZHONG AGRI UNIV
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