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Method for extraction preparation of spirulina platensis soluble polysaccharide

A technology of spirulina polysaccharide and spirulina, applied in the field of sugar engineering, can solve problems such as affecting yield, inability to industrialize production, residues, etc., achieve high polysaccharide extraction rate and polysaccharide purity, the extraction process is simple and easy to operate, and improves high-value utilization. Effect

Inactive Publication Date: 2016-06-08
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these methods all have toxicity and residue, influence the safety of product (as patent publication number is the extraction method of a kind of spirulina polysaccharide of CN103073652A)
There is also a relatively safe protein removal method, such as "a preparation method for spirulina polysaccharide" whose patent publication number is CN103694370A and "a preparation method for spirulina polysaccharide" whose patent publication number is CN103819577A. Exchange resin purification combined with membrane separation technology deproteinization can obtain spirulina polysaccharides with high purity, but the circulating foam separation tower used has high technical requirements and high energy consumption, and the column packing is expensive, and mass production requires more columns , so the production cost is very high, which directly affects the output
In recent years, researchers have begun to explore the enzymatic extraction of polysaccharides from spirulina, but due to the long time-consuming and fragile activity of polysaccharides, it cannot be really used in industrial production.
[0005] How to efficiently break the cell wall of Spirulina is the core of improving the extraction rate of polysaccharides. The conventional methods are hot water extraction, ultrasonic extraction, microwave-assisted extraction, etc., but these methods are not satisfactory in terms of scale output and extraction rate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Take 10g of spirulina algae powder, use water as the extraction solution, stir and dissolve until the spirulina is fully swollen (solid-to-liquid ratio 1:30), homogenize under high pressure under normal temperature conditions, the pressure is 80MPa, the flow rate is 20ml / min, continuous 3 Second-rate.

[0021] 2. The high-pressure homogeneous solution was extracted with 80°C hot water extraction method for spirulina polysaccharides. The extraction time was 4 hours. After extraction, it was centrifuged at 6000rpm for 20 minutes at a high speed to remove the sediment and collect the supernatant.

[0022] 3. Use the sevage method to remove protein, the volume ratio of sevage reagent (chloroform: n-butanol = 4:1) to the above polysaccharide solution is 2:1, shake vigorously for 20 minutes, centrifuge at 6000rpm for 20 minutes, take the upper aqueous phase, and repeat until organic There is no obvious protein layer between layer and aqueous layer.

[0023] 4. After colle...

Embodiment 2

[0025] 1. Take 10g of spirulina algae powder, use water as the extraction solution, stir and dissolve until the spirulina is fully swollen (solid-to-liquid ratio 1:30), homogenize under high pressure under normal temperature conditions, the pressure is 80MPa, the flow rate is 20ml / min, continuous 3 Second-rate.

[0026] 2. The high-pressure homogeneous solution was extracted with 80°C hot water extraction method for spirulina polysaccharides. The extraction time was 4 hours. After extraction, it was centrifuged at 6000rpm for 20 minutes at a high speed to remove the sediment and collect the supernatant.

[0027] 3. Use a flocculant to remove protein. The flocculant is polysilicate ferric sulfate. It is prepared into a 1% solution 24 hours before use, and is added to the above polysaccharide solution according to 1% (v / v) stirring, and the flocculation time is 1h.

[0028] 4. After flocculation, use high-speed centrifugation to remove the sediment. After collecting the supernat...

Embodiment 3

[0030] 1. Take 10g of spirulina algae powder, use water as the extraction solution, stir and dissolve until the spirulina is fully swollen (solid-to-liquid ratio 1:30), homogenize under high pressure under normal temperature conditions, the pressure is 80MPa, the flow rate is 20ml / min, continuous 3 Second-rate.

[0031] 2. The high-pressure homogeneous solution was extracted with 80°C hot water extraction method for spirulina polysaccharides. The extraction time was 4 hours. After extraction, it was centrifuged at 6000rpm for 20 minutes at a high speed to remove the sediment and collect the supernatant.

[0032] 3. Use a flocculant to remove protein. The flocculant is polyphosphorus ferric chloride. It is prepared into a 1% solution 24 hours before use, and added to the above polysaccharide solution according to 1% (v / v) stirring, and the flocculation time is 1h.

[0033] 4. After flocculation, use high-speed centrifugation to remove the sediment. After collecting the supernat...

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PUM

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Abstract

The invention relates to a method for efficient extraction preparation of spirulina platensis soluble polysaccharide. spirulina platensis is taken as the raw material, high pressure homogenization crushing process treatment, hot water extraction, flocculant deproteinization, concentration and precipitation techniques are carried out to obtain spirulina platensis polysaccharide, the polysaccharide purity is more than 95%, and the sugar retention rate reaches 90%. According to the invention, the raw material has abundant sources and is cheap, and the method has the advantages of simple technology, low production cost, green and non-toxic process, and is an effective method for raising the high added value and comprehensive utilization ratio of spirulina platensis.

Description

technical field [0001] The invention relates to polysaccharide extraction technology, in particular to the technology of extracting polysaccharide from algae, belonging to the field of sugar engineering. Background technique [0002] Spirulina (Spirulinaplatensis) is an ancient single-cell species in the ocean. It belongs to Cyanophyta, Oscillatoria, and Spirulina. It is the earliest photosynthetic organism on the earth. It has survived on the earth for 3.5 billion years. And the Food and Agriculture Organization of the United Nations respectively as "the best health food for human beings in the 21st century" and "the most ideal food in the 21st century". Spirulina has a wide ecological distribution, easy cultivation, fast growth, high application value, strong adaptability to changes in temperature and salinity, fast reproduction, easy cultivation, and is suitable for large-scale cultivation. [0003] Spirulina polysaccharides are biological macromolecules present in algae...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 刘启顺尹恒孙永欣陈玮王文霞
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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