Internet and full-automatic double-layer tissue culture room

Abstract

The invention provides an internet and full-automatic double-layer tissue culture room and belongs to the field of plant tissue culture equipment. The room comprises an outer chamber, an inner chamber and a control system, wherein the outer chamber comprises a dressing room and sterilization systems; the inner chamber comprises tissue culture frames, detecting systems, temperature control systems, tissue culture light source systems and illuminating systems; the control system comprises a PLC (programmable logic controller) control system and an upper computer; ultraviolet irradiation sterilization is used for a long time in the outer chamber, a technician can firstly enter the outer chamber for transition when going in or out of the room and puts on sterile clothes before going into the inner chamber, the probability that outside substances adverse to the tissue culture environment are brought into the tissue culture room is reduced to a certain extent, local data and network data are analyzed by means of the control system of the tissue culture room, a tissue culture standard is formed, then, the tissue culture room is uniformly controlled, and real-time, accurate and automatic control can be realized. The internet and full-automatic double-layer tissue culture room provided by the invention has the advantages of being intelligent, high in sterilization efficient and low in environment bacterial density.

Description

technical field [0001] The invention belongs to the field of plant tissue culture equipment, and in particular relates to an Internet+automatic double-layer tissue culture room. Background technique [0002] Tissue culture technology is to place living organs, tissues or cells in the culture medium under sterile conditions and place them in a suitable environment for continuous culture of cells, tissues or individuals. It mainly uses the totipotency of cells, That is, each cell has a complete set of genetic material, and the differentiated plant tissue is induced to dedifferentiate through the special combination of various hormones in the medium, thereby inducing callus, and further inducing new individuals. This technology has been widely used in the research of agriculture, biology and medicine, and has made great contributions to the rapid propagation of plants, the protection of plant species and related research. [0003] However, since the medium used in tissue cultu...

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Problems solved by technology

[0003] However, since the medium used in tissue culture contains a large amount of nutrients, it is not only suitable for the growth of plant cells and tissues, but also suitable for the growth of various bacteria and fungi. The growth of bacteria will consume a large amount of nutrients in the medium, which is harmful to plants. influence on cell growth

In addition, due to the low degree of callus differentiation and poor resistance to bacteria and fungi, it is extremely vulnerable to the substances released by bacteria and fungi, or is poisoned or mutated, which has a great impact on the tissue culture industry. Therefore, a clean and sterile environment is extremely important for tissue culture

[0004] The tissue culture room is an important means to control the tissue culture environment, but the current tissue culture room is mostly an ordinary room. When technicians enter and exit, it is inevitable that bacteria, fungi and other substances that are not conducive to the tissue culture environment will be brought into the tissue culture. Indoors, which affect the effect of tissue culture, and relying on manual monitoring and control of environmental changes in the tissue culture room often cannot make timely adjustments, there will be a certain lag in regulation, and it may be due to human negligence that the plant tissue culture In addition, the growth of plant tissues is complex and changeable, and experienced technicians are often required for tissue culture. The dependence on experienced technicians is too strong, resulting in a significant increase in labor costs, which is not conducive to the development of the tissue culture industry. develop

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