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Livestock synchronous ovulation and fixed-time artificial insemination method

A technology for timed insemination and livestock, applied in the field of animal husbandry, can solve problems such as ovulation control and timed insemination that are not effectively solved, and achieve the effects of increasing the reproductive benefit of dairy cows, accelerating the speed of reproduction, and having broad market application prospects.

Inactive Publication Date: 2016-06-01
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, exogenous hormones such as GnRH, PMSG, FSH, and LH have been applied to the reproductive management of dairy farms, but the problem of combining ovulation control with timed insemination has not been effectively solved. Therefore, there is an urgent need for programmed synchronous ovulation-timed insemination. Fine technology (Ovsynchand fixed-timeArtificialInsemination, OFAI) to solve the above problems

Method used

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  • Livestock synchronous ovulation and fixed-time artificial insemination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 Cow synchronous ovulation-timed insemination method

[0029] 1. Reagents and materials: Chloroprostenol (PGF 2α , Shanghai Institute of Family Planning Science, 0.2mg / bottle); Gona (GnRH, Shanghai Family Planning Science Institute, 100μg / bottle); semen (sperm number 29HO13685, American ABS company); 10mL disposable syringe, long arm Gloves, insemination gun, insemination jacket, tweezers, scissors, thawing cup, thermometer, timer, etc.

[0030] 2. Cattle selection and grouping: Select 82 lactating Holstein cows that are healthy, moderate in body condition (550-650kg / head, body condition score between 2.5-4.0) and 50-85 days postpartum, and randomly divided into two groups. Recorded as test group and control group.

[0031] 3. Synchronous ovulation-timing insemination treatment:

[0032] Experimental group: the cows in this group were injected intramuscularly with GnRH 100 μg for the first time at 15:00-16:00 p.m. on any day of the estrus cycle (day 0), a...

Embodiment 2

[0039] Embodiment 2 Cow synchronous ovulation-timed insemination method

[0040] 1. Reagents and materials: the same as in Example 1.

[0041] 2. Cattle selection and grouping: Select 71 lactating Holstein cows that are healthy, moderate in body condition (550-650kg / head, body condition score between 2.5-4.0) and 50-85 days postpartum, and randomly divided into two groups. Recorded as test group and control group.

[0042] 3. Synchronous ovulation-timing insemination treatment:

[0043] Experimental group: The cows in this group were injected intramuscularly with GnRH 100 μg for the first time at 15:00-16:00 p.m. on any day of the estrus cycle (day 0), and intramuscularly injected PGF at 15:00-16:00 p.m. on the seventh day. 2α 0.4mg, second intramuscular injection of GnRH 100μg at 15:00-16:00 on the ninth day, first timed artificial insemination at 7:00-8:00 am on the tenth day, and 7:00-8:00 am on the eleventh day The second timed artificial insemination.

[0044] Control...

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Abstract

The invention relates to a livestock synchronous ovulation and fixed-time artificial insemination method. The method includes: intramuscularly injecting GnRH (gonadotropin releasing hormone) or analogues thereof to cows in any day of an estrus period, injecting PG (prostaglandin) or analogues thereof after 7-8 days, and injecting the GnRH or the analogues thereof for the second time after 2-3 days. Twice fixed-time artificial insemination in advance or twice fixed-time artificial insemination in delay is carried out respectively according to follicular development conditions of the cows. By regulation of follicular development time of the cows, fixed-time artificial insemination is carried out in relatively fixed estrus time to increase sperm-egg binding probability and the estrus conception rate of the cows, and accordingly reproduction efficiency of the cows is improved. In addition, compared with a conventional OFAI (ovsynch and fixed-time artificial insemination) technology, the livestock synchronous ovulation and fixed-time artificial insemination method has the advantage that the estrus conception rate is increased by 10%-15% due to avoiding of estrus diagnosis before insemination, thereby having promising market application prospect.

Description

technical field [0001] The invention relates to the field of animal husbandry, in particular to a method for synchronous ovulation-timed insemination of livestock. Background technique [0002] The concept of timing insemination of dairy cows was first proposed by Pursley et al. in 1995. Subsequently, researchers carried out a series of studies around the insemination time of cows after hormone treatment. At the same time, due to the influence of various factors, the accuracy of estrus observation and identification of dairy cows is relatively low, which affects the further improvement of the efficiency of timing insemination technology, and it is difficult to achieve 40% conception rate of dairy cows during estrus on large-scale pastures. Therefore, how to control the ovulation time of follicles by injecting exogenous hormones, and then improve the breeding efficiency of dairy cows has become a problem that the majority of dairy cattle breeding enterprises strive to solve....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 郝海生赵明礼朱化彬宋金辉
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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