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Novel application of osthole in preparation of echinococcosis treating drug

A new application technology of osthole, which is applied in the field of new application of Chinese herbal medicine, can solve the problem of treating echinococcosis and other problems that have not been seen before, and achieve the effect of inhibiting growth

Inactive Publication Date: 2016-05-18
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report of using Fructus Cnidii for the treatment of echinococcosis

Method used

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  • Novel application of osthole in preparation of echinococcosis treating drug
  • Novel application of osthole in preparation of echinococcosis treating drug
  • Novel application of osthole in preparation of echinococcosis treating drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Effect of osthole on Echinococcus granulosus:

[0024] Purpose and principle: To observe the survival rate of protoscoleum treated with drugs in vitro by trypan blue staining method, so as to observe the effect of drugs on Echinococcus granulosus. Normal living cells have a complete membrane structure, which can repel trypan blue and prevent it from entering the cell; while cells with inactive or incomplete membranes have increased membrane permeability and can be stained blue by trypan blue color. It is generally considered that the integrity of the cell membrane is lost, that is, it is considered dead.

[0025] Method: The cleaned protoscolm of Echinococcus granulosus was added to the 24-well plate, and the control group and the dosing group were set up. Trypan blue staining was used to record the survival rate of the protoscole.

[0026] The survival rate of the protoscole was calculated according to the following formula: Survival rate = (number of living protosc...

Embodiment 2

[0029] Effect of osthole on anti-hydatid in mice:

[0030] Purpose and principle: To observe the anti-hydatid effect of the drug in vivo by weighing the wet weight of hydatid tissue blocks before and after administration.

[0031] Method: 15 rats were intragastrically administered 100mg / kg per day, and the weight of the tissue pieces was weighed after 6 weeks of administration. Three months after Balb / c female mice were inoculated with hydatid tissues, they were divided into three groups: blank control group, positive control albendazole group, and experimental group osthole group. Each group of five,

[0032] Results and evaluation: if figure 2 As shown, compared with the control group, the wet weight of the tissue block after the action of osthole decreased significantly, indicating that osthole has a good anti-hydatid effect in vivo.

Embodiment 3

[0034] Effects of osthole on cells:

[0035] Purpose and principle: MTTassay was used in the cytotoxicity experiment. MTTassay is an assay that uses a colorimetric method to indirectly measure the number of viable cells. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystalline formazan and deposit in the cells, while dead cells have no such function. Dimethyl sulfoxide can dissolve formazan in cells, and its light absorption value is measured at a wavelength of 490nm with a microplate reader. Within a certain range of cell numbers, the amount of MTT crystal formation is proportional to the number of cells.

[0036] Method: Add HePG-2 and human Zhang's hepatocytes into 96-well plate, set up control group and drug-dosed group, add solvent in the control group, add osthole with different concentrations in the drug-dosed group, add MTT after incubation for 48 hours, Incubate fo...

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Abstract

The invention discloses a novel application of osthole in preparation of an echinococcosis treating drug. The novel application of the osthole in the preparation of the echinococcosis treating drug has the benefits that the application effect of the osthole on the echinococcosis resistance is disclosed for the first time, the application of the osthole is expanded, and one novel echinococcosis treating drug is developed. A cytotoxicity test, an in-vitro echinococcosis protoscolices killing test, an in-vivo hydatid tissue mass growth inhibition test and a drug in-vivo liver and kidney injury test research prove that the osthole has remarkable echinococcosis protoscolices killing effect, can remarkably inhibit growth of hydatid tissue, does not have obvious cytotoxicity when the osthole is in effective concentration and has no influence on liver and kidney functions of a mouse when the osthole is in the effective concentration.

Description

technical field [0001] The invention relates to the technical field of new uses of Chinese herbal medicines, in particular to the new use of osthole in the preparation of medicines for treating echinococcosis. Background technique [0002] Echinococcosis is a serious parasitic disease caused by Echinococcus parasitizing in humans or host animals. my country is a high-incidence area for both cystic and vesicular echinococcosis. At present, the most widely used anti-echinococcosis drug in clinical practice is albendazole, but the intestinal absorption rate of this drug is very poor, and it generally can only inhibit the growth of parasites but cannot completely and effectively kill parasites, so patients must be treated for a long time. Take this medicine in large quantities to achieve therapeutic effect. At the same time, a large number of studies have found that the drug can cause serious adverse drug reactions in multiple systems. Therefore, it is of great significance t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61P33/10
CPCA61K31/37
Inventor 景涛袁苗苗刘焕香郭婧筠辛奇鲁俊李焕平孙旭东那斌高海军宋晓霞
Owner LANZHOU UNIVERSITY
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