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Technology for extracting seaweed protein by means of microbial fermentation method

A microbial fermentation method and seaweed extraction technology, applied in the field of food processing, can solve the problems of inactivation of protein active substances, loss of functional activity, low protein extraction rate, etc.

Inactive Publication Date: 2016-05-18
QINGDAO HAODA MARINE HEALTH FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the extraction process of seaweed protein in my country is still mainly based on alkali extraction and acid extraction. Still need to further improve the technological level in the process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A process for extracting seaweed protein by means of microbial fermentation, the specific preparation steps are as follows:

[0023] (1) Pretreatment: select seaweed without mildew, clean it and crush it through a 40-mesh sieve;

[0024] (2) Enzymatic hydrolysis: Add 10 times the mass ratio of pure water to the pretreated seaweed, adjust the pH to 4.0-5.0, add 3% protease and 2% non-starch polysaccharase in seaweed mass ratio, and heat at 40-60°C Under enzymatic hydrolysis for 2h;

[0025] (3) Preparation of liquid medium: add seaweed mass ratio of 5% molasses, 1.5% ammonium sulfate, and 0.2% potassium dihydrogen phosphate to the enzymatically hydrolyzed seaweed, and adjust the initial pH of the medium to 7.0;

[0026] (4) Activation of Candida utilis: Connect Candida utilis to YPD medium, shake the flask at 30°C and 180r / min for 24 hours; then transfer the seed liquid to YPD at a ratio of 1:100 In the culture medium, the Candida utilis liquid seeds are obtained;

[...

Embodiment 2

[0035] A process for extracting seaweed protein by means of microbial fermentation, the specific preparation steps are as follows:

[0036] (1) Pretreatment: select seaweed without mildew, clean it and crush it through a 40-mesh sieve;

[0037] (2) Enzymatic hydrolysis: Add 10 times the mass ratio of pure water to the pretreated seaweed, adjust the pH to 4.0-5.0, add 2% protease and 1% non-starch polysaccharase in the mass ratio of seaweed, and heat it at 40-60°C Under enzymatic hydrolysis for 3h;

[0038] (3) Prepare liquid medium: add seaweed mass ratio of 3% molasses, 1% ammonium sulfate, and 0.1% potassium dihydrogen phosphate to the enzymatically hydrolyzed seaweed, and adjust the initial pH of the medium to 7.0;

[0039] (4) Activation of Candida utilis: Connect Candida utilis to YPD medium, shake the flask at 30°C and 180r / min for 24 hours; then transfer the seed liquid to YPD at a ratio of 1:100 In the culture medium, the Candida utilis liquid seeds are obtained;

...

Embodiment 3

[0048] A process for extracting seaweed protein by means of microbial fermentation, the specific preparation steps are as follows:

[0049] (1) Pretreatment: select seaweed without mildew, clean it and crush it through a 40-mesh sieve;

[0050] (2) Enzymatic hydrolysis: Add 10 times the mass ratio of pure water to the pretreated seaweed, adjust the pH to 4.0-5.0, add 2.5% protease and 1.5% non-starch polysaccharase in the mass ratio of seaweed, and heat it at 40-60°C Under enzymatic hydrolysis for 2.5h;

[0051] (3) Preparation of liquid medium: add seaweed mass ratio of 4% molasses, 1.2% ammonium sulfate, and 0.15% potassium dihydrogen phosphate to the enzymatically hydrolyzed seaweed, and adjust the initial pH of the medium to 7.0;

[0052](4) Activation of Candida utilis: Connect Candida utilis to YPD medium, shake the flask at 30°C and 180r / min for 24 hours; then transfer the seed liquid to YPD at a ratio of 1:100 In the culture medium, the Candida utilis liquid seeds ar...

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Abstract

In order to overcome the defects in a seaweed protein extracting technology, by means of the microbial fermentation technology, the seaweed protein extracting rate and the water-soluble protein proportion are increased according to seaweed nutritional ingredient changes and the accumulation condition of essential amino acid, lactic acid and other useful metabolites, and therefore the nutritional value of seaweed protein is improved. Seaweed protein is prepared through a technology of compound enzymatic hydrolysis and multi-strain two-step liquid fermentation, multi-bacterium mixed fermentation is adopted, many gene functions in fermentation are added by means of combined strain fermentation, the complex metabolism effect difficult to complete by single strains is completed by combining different metabolic capacities, production efficiency is improved by mean of reciprocity and laterality between microorganisms, and a product is rich in seaweed protein, polypeptide, amino acid, probiotic, vitamin, polysaccharide and other nutritional ingredients.

Description

technical field [0001] The invention relates to a process for extracting seaweed protein by using a microbial fermentation method, which belongs to the field of food processing. Background technique [0002] Seaweed foods include: hair vegetables, seaweed, kelp, sea cabbage, wakame and so on. The annual seaweed production of the global aquaculture industry is 6.5×10 6 t, is mainly developed into food additives and marine vegetable foods. In Asian countries, seaweed is often eaten as a sea vegetable. Japan is the main consumer country, with an average consumption of 1.6kg (dry weight) per person per year. In Europe, seaweed, especially brown seaweed, is mainly used for the production of food additives or meal. [0003] Seaweed has a wide range of uses due to its special composition and functionality. Seaweed contains a certain amount of protein and more polysaccharide cellulose, a small amount of fat, rich in inorganic salts and vitamins. Seaweed contains minerals such ...

Claims

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Application Information

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IPC IPC(8): A23J1/00C12P39/00C12P21/00C12P21/06C12R1/125C12R1/72
CPCA23J1/006C12P21/00C12P21/06C12P39/00
Inventor 徐伦超
Owner QINGDAO HAODA MARINE HEALTH FOOD
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