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PCR primer pair composition for identification or assisted identification of idioplasm of sturgeon, and applications thereof

A technology for assisting identification and composition, which is applied in the directions of recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc. Sturgeon germplasm identification system and other issues

Active Publication Date: 2016-05-11
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since there is no standardized breeding introduction system at present, and there is not yet a complete identification system for sturgeon germplasm at home and abroad, the current situation of sturgeon germplasm is complicated, which is extremely unfavorable for the healthy development of the sturgeon industry.

Method used

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  • PCR primer pair composition for identification or assisted identification of idioplasm of sturgeon, and applications thereof
  • PCR primer pair composition for identification or assisted identification of idioplasm of sturgeon, and applications thereof
  • PCR primer pair composition for identification or assisted identification of idioplasm of sturgeon, and applications thereof

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0101] Embodiment 1, identification sturgeon parent

[0102] The sturgeon germplasm known in Table 2 was used as the sturgeon to be tested to verify the feasibility of identifying the sturgeon germplasm with the composition of PCR primers for identifying or assisting identification of sturgeon germplasm.

[0103] 1. Preparation of PCR primer pair composition for identification or auxiliary identification of sturgeon germplasm

[0104] In this step, the PCR primer pair composition for identifying or assisting identification of the female parent of sturgeon and the male parent of sturgeon is composed of composition A for identifying or assisting identification of the female parent of sturgeon, composition B for identifying or assisting identification of the parent parent of sturgeon and Composition C for identifying or assisting in identifying whether the female sturgeon parent is a sterlet.

[0105] Composition A is made up of a pair of PCR primers called AGF, a pair of PCR pr...

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Abstract

The present invention discloses a PCR primer pair composition for identification or assisted identification of the sturgeon female parent and the sturgeon male parent, and applications thereof. The composition comprises a composition A for identification or assisted identification of the sturgeon female parent, a composition B for identification or assisted identification of the sturgeon parent, and a composition C for identifying or assistedly identifying whether the sturgeon female parent is Acipenser ruthenus, wherein the compositions A, B and C are separately packaged, the composition A comprises AGF, ABF, ABRM, DauF and SchF, the composition B comprises Atr-101, Atr-105, Atr-107, Atr-114, Atr-117, Eu-39, Eu-40, Eu-41, Afu19, Afu22, Afu39, Afu54 and Afu68, and the composition C comprises RutF.

Description

technical field [0001] The invention relates to a PCR primer pair composition for identification or auxiliary identification of sturgeon germplasm in the field of biotechnology and applications thereof. Background technique [0002] Sturgeon belongs to Osteichthyes, Actinopterygii, Chondrostei and Acipenseriformes. Sturgeon is an ancient, economically important, large sub-cold water fish. There are 27 species of sturgeon in 2 families, 6 genera, including 2 species of Huso, 18 species of Acipenser, 2 species of Scaphirhynchus, and Pseudoscaphirhynchus in the family Acipenseridae. ) 3 species, the family Polyodontidae has 1 species each of the genus Psephurus and the genus Polyodon. [0003] While the wild resources of sturgeon are depleting day by day, due to its important economic value, the market demand for sturgeon products is increasing year by year. Therefore, artificial breeding has become the only way to solve the contradiction between the protection of sturgeon re...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 董颖胡红霞朱华郭向贺杨瑞
Owner BEIJING FISHERIES RES INST
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