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Method for identifying varieties of dendrobium huoshanense and dendrobium officinale based on metabonomics

A technology of Dendrobium candidum and metabolomics, applied in the field of analysis and detection of Chinese medicinal materials, to avoid one-sidedness and improve accuracy

Active Publication Date: 2016-05-04
ANHUI AGRICULTURAL UNIVERSITY
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  • Description
  • Claims
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Problems solved by technology

[0005] At present, there is no report on the identification of different Dendrobium species based on metabolomics methods, especially the metabolomics research method based on GC-MS technology to identify different Dendrobium species is unheard of

Method used

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  • Method for identifying varieties of dendrobium huoshanense and dendrobium officinale based on metabonomics
  • Method for identifying varieties of dendrobium huoshanense and dendrobium officinale based on metabonomics
  • Method for identifying varieties of dendrobium huoshanense and dendrobium officinale based on metabonomics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for discriminating Huoshannica and Dendrobium officinale varieties based on metabolomics, comprising the following steps:

[0035] 1. Instruments and reagents

[0036] 1.1 Instrument: Agilent7890A-5975C Gas Chromatography Mass Spectrometer.

[0037] 1.2 Reagents: The cultivated seedlings of Dendrobium officinale and Dendrobium officinale were provided by the College of Life Sciences, Anhui Agricultural University. Methanol and pyridine were of chromatographic grade, and other reagents were of analytical grade.

[0038] 2. Preparation of Dendrobium metabolomics samples:

[0039] 2.1 Extraction of metabolites from dendrobium samples

[0040] The leaves of the annual Huoshanense origami and Huoshanense Dendrobium cultivation seedlings were taken as experimental materials, which were ground to powder by liquid nitrogen grinding method, and each Dendrobium sample was taken for six replicates. Then weigh 100-150 mg of Dendrobium sample and place it in a 10 mL cent...

Embodiment 2

[0052] Effect of different derivatization reaction conditions on the detection of Dendrobium metabolomics

[0053] 1. Instruments and reagents

[0054] 1.1 Instrument: Agilent7890A-5975C Gas Chromatography Mass Spectrometer.

[0055] 1.2 Reagents: Huoshan Dendrobium officinale seedlings were provided by the College of Life Sciences, Anhui Agricultural University. Methanol and pyridine were of chromatographic grade, and other reagents were of analytical grade.

[0056] 2. Effects of different derivatization reaction conditions on the detection of Dendrobium metabolomics

[0057] The test is divided into four groups, and the four groups are all based on the method similar to Example 1 to obtain the annual leaf methanol / water phase metabolomics sample of Dendrobium officinale. The only difference lies in the derivatization reaction conditions. The derivatization reaction conditions of the four groups of experiments in this embodiment are shown in Table 2 below.

[0058] Deriva...

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Abstract

The invention discloses a method for identifying the varieties of dendrobium huoshanense and dendrobium officinale based on metabonomics. The method comprises the following specific steps: (1) collecting dendrobium samples; (2) preparing dendrobium metabonomics samples; (3) detecting metabolites of the dendrobium samples; and (4) performing metabonomics data processing and analysis. The method provided by the invention has the following advantages: a lot of endogenous metabolite information of dendrobium is acquired by means of metabonomics; among the endogenous metabolites, the characteristic metabolites for identification of dendrobium huoshanense and dendrobium officinale are selected out, and then such two dendrobium varieties are distinguished from each other on the whole; the technical mode is advanced and the experimental result is reliable; and a good means is provided for dendrobium variety identification and dendrobium quality assessment.

Description

technical field [0001] The invention relates to the field of analysis and detection of traditional Chinese medicinal materials, in particular to a method for distinguishing Huoshan officinale and Huoshan officinale varieties based on metabolomics. Background technique [0002] Dendrobium is a commonly used precious Chinese medicinal material, which has the functions of nourishing yin and clearing heat, benefiting stomach and promoting body fluid, clearing sound and improving eyesight, reducing inflammation and relieving pain. The phytochemical composition of Dendrobium is complex, containing polysaccharides, alkaloids, phenanthrenes, bibenzyls, amino acids and other medicinal active ingredients. However, due to the rich species of Dendrobium and high morphological similarity, the sources of Dendrobium in the market are complicated, and the phenomenon of shoddy and fake ones often occurs, which has a serious impact on the clinical efficacy and drug safety of Dendrobium. [0...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/067
Inventor 金青焦春燕孙时伟蔡永萍林毅宋程李姝妹王燕
Owner ANHUI AGRICULTURAL UNIVERSITY
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