Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography

A high-performance liquid chromatography, livestock and poultry manure technology, applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of few types of antibiotics and high cost of antibiotic detection methods, and achieve the effects of low detection cost and high detection efficiency.

Active Publication Date: 2016-05-04
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
View PDF5 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings and deficiencies of the antibiotic detection method in the prior art, such as high cost, few types of antibiotics, and staying in the field of animal food detection, the present invention provides a method for rapidly detecting various veterinary antibiotics by using high performance liquid chromatography

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography
  • Method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography
  • Method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention will be further described in detail below in conjunction with examples, but the embodiments of the present invention are not limited thereto.

[0020] The instrument conditions of the method for simultaneously detecting multiple antibiotics in livestock and poultry manure by high performance liquid chromatography are as follows:

[0021] The detector is a variable wavelength scanning ultraviolet detector (VWD), the detection wavelength is 274nm, the column temperature is 35°C, the flow rate is 0.6mL / min, the injection volume is 20μL, the mobile phase A is 0.1% formic acid solution, and the mobile phase B is methanol / Acetonitrile was mixed according to v / v ratio of 1:1, gradient elution sequence: 0~18min, 80%A; 18~20min, 80%~40%A; 20~30min, 40%A; 30~32min, 40%~ 80%A; 32~35min, 80%A.

[0022] Separation and monitoring of 11 kinds of antibiotic residues in pig manure:

[0023] (1) The instrument used is Agilent 1200 high performance liquid chromato...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an antibiotic detection method, and in particular, relates to a method for simultaneously detecting various antibiotics in livestock and poultry manure by high performance liquid chromatography; the method comprises the steps: pretreating a livestock and poultry manure sample, to obtain a to-be-detected liquid; performing analyte separation of the to-be-detected liquid by a high performance liquid chromatograph, and calculating peak areas corresponding to separated residual antibiotics; and quantitatively calculating according to a standard curve, to obtain the residual content. The method is applied in detection of 11 kinds of residual antibiotics in the livestock and poultry manure; compared with a high performance liquid chromatography-mass spectrometry coupled technique, the method has the advantages of lower detection cost and high detection efficiency, can simultaneously separate 11 kinds of antibiotics, has analytes without interference of major interferents, and basically does not affect qualitative and quantitative analysis of the analytes.

Description

technical field [0001] The invention relates to an antibiotic detection method, in particular to a method for simultaneously detecting multiple antibiotics in livestock and poultry feces by using high performance liquid chromatography. Background technique [0002] Antibiotics refer to substances with the same or similar structure that are obtained from bacteria, actinomycetes, fungi and other microorganisms through cultivation or synthesized by chemical synthesis, and have the ability to kill or inhibit pathogenic microorganisms. In recent years, a large number of antibiotics have been widely used in livestock and poultry breeding as veterinary drugs and feed additives, and play an important role in the prevention and control of animal diseases, improving feed conversion efficiency, and promoting animal growth and development. However, the antibiotics that enter the animal body through oral or intramuscular injection cannot be completely absorbed and utilized, and the amoun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34
CPCG01N30/02G01N30/34G01N2030/027
Inventor 李兆君冯瑶张文娟
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com