A kind of liquid photosynthetic bacteria high vigor preservation method

A technology of photosynthetic bacteria and preservation methods, applied in the direction of microorganism-based methods, biochemical equipment and methods, preservation of microorganisms, etc., can solve the problems of increasing production processes and production costs, not suitable for large-scale production, and large loss of vitality, etc., to achieve Eliminate the risk of secondary pollution, good vitality preservation effect, and low preservation cost

Inactive Publication Date: 2020-09-01
南宁海关技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to promote the application of photosynthetic bacteria, experts and scholars have studied a variety of immobilization technologies, such as "immobilization method of photosynthetic bacteria" (public number CN1810966A), "a preparation method of solid photosynthetic bacteria" (public number CN104293765A), " Solid-state photosynthetic bacteria technology" (publication number CN1124293), etc., but the above-mentioned technologies are mainly to solve the transportation problem. In actual operation, not only the production process and production cost are increased, but some vitality loss is also very large, which is not suitable for large-scale production. In addition, the fermentation liquid discharged during the immobilization process is also likely to cause secondary pollution, which is also an important reason why liquid photosynthetic bacteria are still the main body in the market

Method used

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  • A kind of liquid photosynthetic bacteria high vigor preservation method

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Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of liquid photosynthetic bacteria comprises the following steps:

[0034] (1) Preparation of seed culture solution: weigh the following components in parts by weight: 3 parts of sodium acetate, 1 part of sodium bicarbonate, 1 part of ammonium chloride, 0.5 parts of dipotassium hydrogen phosphate, 0.2 parts of magnesium chloride, Ferrous-EDTA 0.005 parts, sodium chloride 5 parts, distilled water 1000 parts, mix well, adjust the pH value to 7.4±0.2, steam sterilize at 121°C for 15min, cool to 30°C, and set aside;

[0035](2) Preparation of expanded culture medium: Weigh the following components in parts by weight: 4 parts of sodium acetate, 1.5 parts of sodium bicarbonate, 1.5 parts of ammonium chloride, 0.6 parts of dipotassium hydrogen phosphate, 0.25 parts of magnesium chloride, Ferrous-EDTA 0.006 parts, sodium chloride 6 parts, drinking water 1100 parts, mix well, adjust the pH value to 7.4±0.2, steam sterilize at 121°C for 15min, cool to 30°C, and set...

Embodiment 2

[0040] The preparation of liquid photosynthetic bacteria comprises the following steps:

[0041] (1) Preparation of seed culture solution: Weigh the following components in parts by weight: 2 parts of sodium acetate, 1.5 parts of sodium bicarbonate, 0.5 parts of ammonium chloride, 0.6 parts of dipotassium hydrogen phosphate, 0.25 parts of magnesium chloride, Ferrous-EDTA 0.004 parts, sodium chloride 5 parts, distilled water 900 parts, mix well, adjust the pH value to 7.4±0.2, steam sterilize at 121°C for 15min, cool to 30°C, and set aside;

[0042] (2) Preparation of expanded culture medium: Weigh the following components in parts by weight: 3 parts of sodium acetate, 1 part of sodium bicarbonate, 1 part of ammonium chloride, 0.6 part of dipotassium hydrogen phosphate, 0.15 part of magnesium chloride, 0.15 part of magnesium chloride, Ferrous-EDTA 0.005 parts, sodium chloride 4 parts, drinking water 950 parts, mix well, adjust the pH value to 7.4±0.2, steam sterilize at 121°C f...

Embodiment 3

[0047] The preparation of liquid photosynthetic bacteria comprises the following steps:

[0048] (1) Preparation of seed culture solution: Weigh the following components in parts by weight: 4 parts of sodium acetate, 0.5 parts of sodium bicarbonate, 1.5 parts of ammonium chloride, 0.4 parts of dipotassium hydrogen phosphate, 0.2 parts of magnesium chloride, Ferrous-EDTA 0.006 parts, sodium chloride 4 parts, distilled water 1000 parts, mix well, adjust the pH value to 7.4±0.2, steam sterilize at 121°C for 15min, cool to 30°C, and set aside;

[0049] (2) Preparation of expanded culture medium: Weigh the following components in parts by weight: 3 parts of sodium acetate, 1 part of sodium bicarbonate, 1 part of ammonium chloride, 0.5 parts of dipotassium hydrogen phosphate, 0.2 parts of magnesium chloride, Ferrous-EDTA 0.005 parts, sodium chloride 5 parts, drinking water 1000 parts, mix well, adjust the pH value to 7.4±0.2, steam sterilize at 121°C for 15min, cool to 30°C, and set...

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Abstract

The invention discloses a high-activity preservation method of liquid PSB (photosynthetic bacteria). The process of the preservation method is as follows: collagen is added to to-be-preserved liquid PSB, then the pH value of the liquid PSB is adjusted to range from 6.8 to 7.5 by the aid of a phosphate buffer solution, and then the liquid PSB are preserved in a sealed manner. The method is simple to operate, low in cost, easy to implement and good in environmental benefit, preservation condition requirements are simple, the liquid PSB can be placed indoors under natural conditions for preservation, the activity of the liquid PSB can be maintained effectively for a long time, the effective living bacterium count is higher than 90% after 6 months of preservation, the effective living bacterium count is higher than 80% after 12 months of preservation, activation is not required during application, effects can be realized immediately, and the preservation method of the liquid PSB is worthy of vigorous popularization and application.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, in particular to a method for long-term preservation of high vigor of liquid photosynthetic bacteria. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria, referred to as PSB) are the earliest prokaryotic organisms with a primitive light energy synthesis system on the earth. They are widely distributed in oceans, lakes, rivers, paddy fields, sludge and soil. Molecules such as ammonia nitrogen, organic acids, etc. are used as growth factors to carry out non-oxygenous photosynthesis of bacteria, which belong to the bacterial phylum, Eubacteria, and Rhodospirales in classification, according to "Bergey's Bacteria Identification Manual" (Ninth Edition) It can be divided into 6 taxa and 27 genera. The six taxa are Chromobacteriaceae, Exothorhodospiraceae, Purple Non-sulfur Bacteria, Green Sulfur Bacteria, Multicellular Chlorothycium, and Halobacteria. [0003...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/04C12R1/01
CPCC12N1/04
Inventor 刘军义罗兆飞罗佳刘鹏扬吕春秋盘宝进陈立军
Owner 南宁海关技术中心
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