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A kind of preparation method of peanut nano-peptide

A nano, peanut technology, applied in the direction of plant protein processing, application, protein food ingredients, etc., to reduce the cost of enzymatic hydrolysis and improve the efficiency of enzymatic hydrolysis

Active Publication Date: 2019-06-25
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-pressure microfluidic treatment has become an effective method to improve the functional properties of proteins, but the research on protein modification by high-pressure microfluidic to facilitate the preparation of short peptides has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Step 1: Weigh 150g of peanut protein powder, add 1500mL of deionized water to prepare a 10% (w / v) concentration solution, adjust the pH value to 8.0, stir and dissolve for 120min, and centrifuge at 4000rpm for 15min; take the supernatant and adjust the pH value to 4.5, set aside for 60 minutes; centrifuge at 4000rpm for 15 minutes; take the precipitate, add water to dissolve, adjust the pH value to 7.0, stir and dissolve for 120 minutes; spray dry to obtain peanut protein isolate;

[0052] Step 2: Weigh 60g of the spray-dried peanut protein isolate, add 1000mL of deionized water to prepare a 6% (w / v) solution, stir and dissolve for 60min; turn on the micro-jet ultra-high pressure homogenizer, and take 100mL peanut protein isolate solution , without applying pressure, put it into the equipment, take the effluent, as a control; then, take samples of 100mL solution in turn as 7 different pressure treatment groups, respectively at 30MPa, 60MPa, 90MPa, 120MPa, 150MPa, 180MPa,...

Embodiment 2

[0054] Example 2 Effects of different pressure treatments on the molecular weight and ACE inhibitory activity of peanut short peptides.

[0055] Step 1: Weigh 300g of peanut protein powder, add 3000mL of deionized water to prepare a 10% (w / v) concentration solution, adjust the pH value to 8.0, stir and dissolve for 120min, and centrifuge at 4000rpm for 15min; take the supernatant and adjust the pH value to 4.5, set aside for 60 minutes; centrifuge at 4000rpm for 15 minutes; take the precipitate, add water to dissolve, adjust the pH value to 7.0, stir and dissolve for 120 minutes; spray dry to obtain peanut protein isolate;

[0056] Step 2: Weigh 120g of the spray-dried peanut protein isolate, add 2000mL of deionized water to prepare a 6% (w / v) solution, stir and dissolve for 60min; turn on the micro-jet ultra-high pressure homogenizer, and take 150mL of the peanut protein isolate solution , without applying pressure, put it into the equipment, take the effluent, as a control; ...

Embodiment 3

[0064] Example 3 The influence of high-pressure micro-fluidic treatment and the concentration of the control enzymatic hydrolysis material.

[0065] Step 1: Weigh 100g of peanut protein powder, add 1000mL of deionized water to prepare a 10% (w / v) concentration solution, adjust the pH value to 8.0, stir and dissolve for 120min, and centrifuge at 4000rpm for 15min; take the supernatant and adjust the pH value to 4.5, set aside for 60 minutes; centrifuge at 4000rpm for 15 minutes; take the precipitate, add water to dissolve, adjust the pH value to 7.0, stir and dissolve for 120 minutes; spray dry to obtain peanut protein isolate;

[0066] Step 2: Weigh 45g of the spray-dried peanut protein isolate, add 750mL of deionized water to prepare a 6% (w / v) solution, stir and dissolve for 60min; turn on the micro-jet ultra-high pressure homogenizer, and take 750mL of the peanut protein isolate solution , Circulate 3 times under the pressure of 120MPa, and take the effluent. Dry the efflu...

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Abstract

The invention discloses a method for preparing a peanut nano peptide with high ACE inhibition activity, which adopts a high-pressure micro-jet method to physically modify peanut protein so as to improve enzymolysis efficiency. The method comprises the following steps: preparing peanut isolated protein; carrying out high-pressure micro-jet treatment; carrying out continuous enzymolysis on neutral protease and compound protease; and carrying out enzyme deactivation, centrifuging, carrying out ultrafiltration and drying to obtain the peanut nano peptide with the high ACE inhibition activity. With the adoption of the method disclosed by the invention, the enzymolysis efficiency is improved and the production cost is reduced; the grain diameter of the obtained peanut nano peptide is 14.4nm; and an ACE inhibition IC50 value is 0.192mg / mL, and the good ACE inhibition activity is represented.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of peanut nanopeptide. Background technique [0002] Peanut protein is an important protein source for preparing short peptides with excellent physiological activity. Some peanut protein hydrolysates, tripeptides, tetrapeptides, and hexapeptides have been found to have good ACE inhibitory activity, but ACE inhibitory monomer peptides need to undergo High-cost, low-yield enzymatic hydrolysis and purification process, so the practical application is limited. Improving the efficiency of enzymatic hydrolysis, fully excavating a large number of high physiologically active short peptides contained in peanut protein, at the same time appropriately reducing production costs, and improving the application of mixed peanut short peptides in functional foods have become an important development direction of peanut short peptides. Physical modification of protein...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J3/34A23J3/14
CPCA23J3/14A23J3/346A23V2002/00A23V2250/55
Inventor 王强石爱民龚魁杰刘红芝刘丽胡晖杨颖
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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