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Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method

The technology of Polygonatum polyflora and a new method is applied in the directions of horticultural methods, botanical equipment and methods, horticulture, etc., and can solve the problems of unfavorable cultivation scale standard promotion, accumulation of vegetatively propagated tuber virus, low reproduction coefficient, etc. The effect of neat growth and high survival rate

Inactive Publication Date: 2016-05-04
福建柘参生物科技研究股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a new method for large-scale cultivation of Polygonatum polyflora with high yield and high quality, which overcomes the problems of low reproduction coefficient and large amount of rhizomes in propagation of Polygonatum polyflora at present, which is not economical, but also limits the scale, and the accumulation of asexual tuber viruses accumulates all the year round. The degradation of seedlings is not conducive to the standardization and popularization of cultivation scale. Therefore, the present invention provides a new method of large-scale cultivation of Polygonatum polyflora with high yield and high quality, which is of high quality, low cost, and can quickly obtain a large number of virus-free seedlings.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0014] Embodiment 1: a kind of high-yield and high-quality large-scale cultivation method of Polygonatum multiflora, comprising the steps of:

[0015] (1) Acquisition and treatment of explants: Take the ungerminated new shoots on Polygonatum multiflora tubers, peel off 2-3 layers of outer coverings in the laboratory, place them under running water, wash them with running water for 2 hours, and put them in a sterilized small beaker Transfer to the ultra-clean bench;

[0016] (2) Sterile seedling induction: The explants treated in step (1) were sterilized with 70% ethanol for 30 seconds, washed twice with sterile water, and then sterilized in 0.1% mercuric chloride for 8 minutes. Wash 5 times with sterile water, take 0.5mm crystalline shoot tip cell mass under stereoscopic environment, transfer the shoot tip to MS+4.0mg / LBA+0.4mg / LNAA medium for culture, add 3 % sucrose, 0.5% agar, pH is 5.8, and the culture conditions are controlled, the culture conditions are: temperature 25°...

Embodiment 2

[0021] Embodiment 2: a kind of high-yield and high-quality large-scale cultivation method of Polygonatum multiflora, comprising the steps of:

[0022] (1) Acquisition and treatment of explants: Take the ungerminated new shoots on Polygonatum multiflora tubers, peel off 2-3 layers of outer coverings in the laboratory, place them under running water, wash them with running water for 2 hours, and put them in a sterilized small beaker Transfer to the ultra-clean bench;

[0023] (2) Sterile seedling induction: The explants treated in step (1) were sterilized with 70% ethanol for 30 seconds, washed twice with sterile water, and then sterilized in 0.2% mercuric chloride for 8 minutes. Wash 5 times with sterile water, take 0.5mm crystalline shoot tip cell mass under stereoscopic environment, transfer the shoot tip to MS+4.0mg / LBA+0.4mg / LNAA medium for culture, add 3 % sucrose, 0.5% agar, pH is 5.8, and the culture conditions are controlled. The culture conditions are: temperature 25°...

Embodiment 3

[0028] Embodiment 3: a kind of high-yield and high-quality large-scale cultivation method of Polygonatum multiflora, comprising the steps of:

[0029] (1) Acquisition and treatment of explants: Take the ungerminated new shoots on Polygonatum multiflora tubers, peel off 2-3 layers of outer coverings in the laboratory, place them under running water, wash them with running water for 2 hours, and put them in a sterilized small beaker Transfer to the ultra-clean bench;

[0030](2) Sterile seedling induction: The explants treated in step (1) were sterilized with 70% ethanol for 30 seconds, washed twice with sterile water, and then sterilized in 0.15% mercuric chloride for 8 minutes. Wash 5 times with sterile water, take 0.5mm crystalline shoot tip cell mass under stereoscopic environment, transfer the shoot tip to MS+4.0mg / LBA+0.4mg / LNAA medium for culture, add 3% sucrose, 0.5% agar, pH 5.8, control culture conditions, the culture conditions are: temperature 25°C, culture in compl...

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Abstract

The invention discloses a novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method and relates to the technical field of traditional Chinese medicine cultivation. The novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method comprises the six steps of explant obtaining and processing, aseptic seedling induction, tuber propagation, tuber adventitious-bud induction and strong seedling rooting, polygonatum cyrtonema seedling acclimatization and polygonatum cyrtonema seedling transplantation in fields. The novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method establishes an intermediate polygonatum cyrtonema propagation system, and a large amount of detoxified polygonatum cyrtonema seedlings can be quickly obtained; the survival rate of transplanted tuber seedlings is high, and the seedlings grow fast in a later period; the emerged polygonatum cyrtonema seedlings grow in order, do not easily fall ill and are excellent in quality, management is simplified, the cost is low, and the cultivation method has popularization value.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine cultivation, in particular to a new large-scale cultivation method of Polygonatum multiflora with high yield and high quality. Background technique [0002] Polygonatum multiflora is a perennial herb of the Liliaceae Polygonatum, which has medicinal, edible, ornamental and beauty value. There are many kinds of sealwort, among which Polygonatum multiflora is the best in quality. Polygonatum multiflora rhizome is rich in sugars, steroidal saponins, cardiac glycosides, alkaloids, flavonoids and anthraquinones, lignans, vitamins, amino acids and trace elements. It is a traditional bulk medicinal material in my country. It has the functions of invigorating qi and nourishing yin, invigorating the spleen, nourishing the lungs, nourishing the kidney, and inhibiting tumor cells. It has very important development and utilization value, and has broad prospects in the development of new d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 鞠建文游永铃苏志杭叶祖云刘群
Owner 福建柘参生物科技研究股份有限公司
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