An ultra-clean high-stability biological 3D printing-culturing integrated system and a method therefor

Abstract

The invention provides an ultra-clean high-stability biological 3D printing-culturing integrated system and a method therefor, and overcomes problems namely environment pollution and equipment corrosion which are caused by separation of biological printing from culturing systems. The integrated system can achieve synchronous printing of various cells and cytoskeletons in a biological printing system, namely integration of 3D printing and culturing. Cell activity in a printing process and functions and differentiation of printed cells can be ensured and are protected from being influenced by outer environments. The integrated system adopts a design that culturing and printing driving are separated. Printing nozzles and a clamp are embedded in the biological culturing system, and therefore a sterile culture environment is protected from being damaged by printing equipment and the outer environment on the basis of ensuring barrier-free printing, and integration of high-precision cell printing and culturing is achieved. Special requirements of biological culturing temperature and humidity on materials, dimension, designs, and the like of workbench components are reduced through the integrated system. The integrated system improves positioning precision of a workbench, increases the speed, and the like, prolongs the service lifetime of a driving module, and greatly reduces the cost.

Description

technical field [0001] The invention belongs to the field of 3D printing and tissue engineering, and in particular relates to an ultra-clean and high-stability biological 3D printing and culture integration system and method. Background technique [0002] The 3D printing of cell scaffolds and the in vitro culture of printed tissues are the two most critical aspects in the entire field of 3D bioprinting. Most of the traditional biological tissue printing is that printing and culturing are two independent parts. After printing, they are transferred to the corresponding culturing environment. Due to the large difference between the environment and the culturing environment during the printing and transfer process, it cannot be achieved. The requirements of the culture environment reduce the printing quality and survival rate of cells. At present, some methods of combining printing and culturing have been used, and the printing table is directly placed in the culturing environm...

AI Technical Summary

Problems solved by technology

However, the limited cultivation space and high cultivation temperature and humidity requirements will increase the requirements for workbench component materials, design dimensions and motion strokes

Anti-corrosion of materials and lubrication of screw bearings will pollute the environment

Even if a material that can adapt to the corresponding environment is found, its mechanical properties, service life, ease of processing, material cost and non-toxicity are difficult to meet the requirements at the same time

Images