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Artificial breeding method for symbiotic germchit of Dendrobium devonianum

A Dendrobium dendrobium, artificial breeding technology, applied in botany equipment and methods, planting substrates, gardening, etc., can solve problems such as failure

Active Publication Date: 2016-04-27
JINGHONG HONGZHEN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no successful report in the prior art

Method used

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  • Artificial breeding method for symbiotic germchit of Dendrobium devonianum
  • Artificial breeding method for symbiotic germchit of Dendrobium devonianum
  • Artificial breeding method for symbiotic germchit of Dendrobium devonianum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Acquisition and preservation of tooth lobe germplasm

[0041] The seeds of Dendrobium dendrobii that are naturally strong and mature but not cracked in the field are collected and preserved for future use. Dendrobium dendrobium seeds used were collected from wild populations in Longling County, Baoshan City, Yunnan Province.

[0042] 2. Preparation of fungal suspension

[0043] 2.1 Production of medium: PDA liquid medium is conventionally used, with an appropriate amount. Put 50ml of each bottle into 70 bottles of 500ml Erlenmeyer flasks, wrap the mouth of the bottle with newspaper after sealing, put it into a sterilizer and sterilize it at 121°C and 115KPa for 20min, and set it aside.

[0044] 2.2 Inoculate the strain Tulasnella sp. CGMCC No.9551, which was activated and cultured for 7 days, into the above-mentioned sterilized Erlenmeyer flask containing 50 ml of liquid medium, and ferment and culture it at 28°C and 150 rpm for 7 days.

[0045] 2.3 Filter the cul...

Embodiment 2

[0065] Repeat Example 1, with the following differences:

[0066] From January to February 2015, a simple seedbed was built in the open space of the Scientific Research Center of Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, and the 1m high space above the seedbed was shaded with a shading net with a shading degree of 75%. The seedbed covers an area of About 9 square meters. The average temperature ranges from 13°C to 24°C.

[0067] Get 100ml prepared seed-fungus suspension and spray evenly on the seedling trays equipped with substrate with a watering can, the seed density of the suspension is 185 / ml, sow 4 seedling trays altogether. At the same time, the agar-seed suspension was sprayed as a control experiment, the seed density was 320 / ml, and the control seedling tray was placed more than 30m away from the co-growth seedling tray to prevent fungal contamination, and 2 seedling trays were sown. The rest of the culture conditions were the same.

[00...

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Abstract

The invention discloses an artificial breeding method for symbiotic germchit of Dendrobium devonianum. The method comprises the steps of subjecting Tulasnella sp., of which the collection number is CGMCC No. 9551, to amplified fermenting culture, filtering mycelia, adding a proper volume of sterile water into the mycelia, carrying out crushing by a refiner so as to prepare a fungus suspension, adding Dendrobium devonianum seeds into the fungus suspension so as to prepare a seed-fungus suspension, preparing a seedling raising matrix from coco coir, tree leaves and tree barks, sprinkling 50-500ml of the seed-fungus suspension to the matrix per square meter, and carrying out breeding for 45 days while maintaining the moisture content of the seedling raising matrix to 50-90%, thereby obtaining a large number of symbiotic young seedlings. According to the method, the seedling forming time of the seeds can be remarkably shortened, the seedling raising cost can be reduced, a relatively large number of Dendrobium devonianum young seedlings can be obtained in one time, and the growth speed and natural-environment-transplanted survival rate of the symbiotic young seedlings are improved and are higher than those of asymbiotic-germinated young seedlings. The method achieves a new way for obtaining the seed seedlings from the Dendrobium devonianum seeds and has important practical popularization and application values.

Description

technical field [0001] The invention belongs to the technical field of dendrobium cultivation, and in particular relates to an artificial breeding method for rapidly obtaining a large amount of symbiotic seedlings of dendrobium dendrobium by using fungi to treat dendrobium dendrobium seeds. Background technique [0002] As a traditional Chinese medicinal material, Dendrobium devonianum has extremely high medicinal value. Its cultivation scale is second only to Dendrobium candidum, and it has even become a pillar industry in some areas (such as Longling County, Yunnan Province). At present, the main cultivation modes of Dendrobium include the intensive planting of enterprises and the "company + base + farmer" mode adopted by enterprises, and the small-scale intensive planting by some small enterprises or farmers using simple scaffolding, and the imitation planting by a small number of enterprises or individuals. ecological planting. The seedling sources of various cultivatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00C12N1/14
CPCA01G24/00A01G31/00C12N1/14
Inventor 邵士成高江云刘强黄晖
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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