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Preparation method for blood cell protein peptide chelated calcium microcapsule preparation

A technology of hemoglobin and chelated calcium, which is applied in the field of bioengineering, can solve the problems of pollution and low effective utilization rate, and achieve the effects of reducing environmental pollution, increasing utilization rate and added value, and improving immunity

Inactive Publication Date: 2016-04-20
桐城市雨润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Domestic pig blood resources are very rich, known as liquid meat, its amino acids are comprehensive, but the effective utilization rate is not high, except for a few edible or spray-dried into blood plasma protein powder and blood blood protein powder, most of them are discharged into the surrounding environment In the middle, there is a lot of pollution, and there are few high value-added products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A preparation method of hemoglobin peptide chelated calcium microcapsule preparation, the specific steps are as follows:

[0029] (1) Pretreatment of blood cells: Dissolving a certain amount of pig blood cells in deionized water to make hemolysis, the weight ratio of hemolysis to deionized water is 1:1.5; then use 6MNaOH to adjust the pH value of hemolysis to 7.0;

[0030] (2) Protease enzymatic hydrolysis: add compound animal protease into hemolysis, heat up to 55°C and continue to stir the enzymolysis reaction for 6 hours, wherein, the amount of protease added is 0.8% of the original pig blood cell weight; the compound animal protease used is Guangxi Provided by Nanning Pangbo Biological Engineering Co., Ltd.

[0031] (3) Enzyme inactivation and pressure filtration: heat the enzymatic hydrolysis solution to 85°C, keep it for 25 minutes to inactivate the enzyme, then adjust the pH value of the enzymatic hydrolysis solution to 5.0 with hydrochloric acid after cooling do...

Embodiment 2

[0036] A preparation method of hemoglobin peptide chelated calcium microcapsule preparation, the specific steps are as follows:

[0037] (1) Pretreatment of blood cells: dissolve a certain amount of pig blood cells in deionized water to make hemolysis, the weight ratio of hemolysis to deionized water is 1:3; then use 6MNaOH to adjust the pH value of hemolysis to 9.0;

[0038] (2) Protease enzymatic hydrolysis: Add alkaline protease to hemolysis, heat up to 58°C and continue stirring for 2 hours, then add 1398 neutral protease and flavor protease, do not adjust the pH, adjust the temperature to 50°C and continue stirring Enzymolysis reaction 4h, wherein, the addition amount of alkaline protease is 1.5% of the original pig blood cell weight, the addition amount of 1398 neutral protease is 1% of the original pig blood cell weight, the addition amount of flavor protease is 1% of the original pig blood cell weight %. The alkaline protease, neutral protease and flavor protease used...

Embodiment 3

[0044] A preparation method of hemoglobin peptide chelated calcium microcapsule preparation, the specific steps are as follows:

[0045] (1) Pretreatment of blood cells: dissolve a certain amount of pig blood cells in deionized water to make hemolysis, the weight ratio of hemolysis to deionized water is 1:4; then use 6MNaOH to adjust the pH value of hemolysis to 8.5;

[0046] (2) Protease enzymatic hydrolysis: Add alkaline protease to hemolysis, heat up to 55°C and continue stirring for 1 hour, then add 1398 neutral protease and flavor protease, do not adjust the pH, adjust the temperature to 50°C and continue stirring Enzymolysis reaction 3h, wherein, the addition amount of alkaline protease is 1.5% of the original pig blood cell weight, the addition amount of 1398 neutral protease is 1.5% of the original pig blood cell weight, the addition amount of flavor protease is 1% of the original pig blood cell weight %. The alkaline protease, neutral protease and flavor protease use...

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Abstract

The invention relates to a preparation method for a blood cell protein peptide chelated calcium microcapsule preparation. The preparation method comprises the specific steps that pig blood cells and deionized water are prepared into hemolytic blood, then protease is added to the hemolytic blood, enzyme deactivation and filter pressing are carried out after hydrolysis, and the mixture carries out a chelation reaction with soluble calcium salt after the pH is adjusted; then a proper wall material solution is adopted for embedding the protein peptide chelated calcium solution, and blood cell protein peptide chelated calcium microcapsules are obtained through spray drying after embedding. The prepared blood cell protein peptide chelated calcium microcapsules are high in chelation rate and embedding rate, the solubility of calcium salt in the gastrointestinal tract environment can be improved, and the slow release effect can be achieved. In addition, after calcium salt is released, the absorption rate of calcium is increased through the form of small peptide chelated calcium, the preparation process is safe and free of pollution, absorption of mineral element calcium is promoted while essential amino-acid for the human body is supplemented, and the blood cell protein peptide chelated calcium microcapsule preparation is a good nutrient supplement product.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a preparation method of hemoglobulin peptide chelated calcium microcapsule preparation. Background technique [0002] At present, the first, second and third generation calcium preparations used to supplement human minerals all have disadvantages in different degrees. The first generation calcium preparations are mostly inorganic calcium. Although they have high calcium content, convenient production and low price, their absorption rate is low Low and easy to cause gastrointestinal side effects; the second-generation calcium preparations are traditional organic calcium such as calcium lactate, calcium gluconate, etc., its solubility is better than inorganic calcium, but it is easy to react with phytate and oxalate in food and lead to utilization The rate is low, and the accumulation of acid radicals of organic calcium salts in the body will cause adverse reactions; altho...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A23L33/18A23L33/165A61K38/00A61P3/00
CPCA23V2002/00A61K38/00C12P21/06A23V2200/30
Inventor 崔群维杨风玲宁发子周全陈甲超贾金丽王洪武其他发明人请求不公开姓名
Owner 桐城市雨润生物科技有限公司
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