Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Yarrowia lipolytica and method for producing gamma-decalactone by using same

A technology of Yarrowia lipolytica and decalactone, which is applied in the direction of microbial-based methods, biochemical equipment and methods, and bulk chemical production, to achieve the effects of increasing yield, good economic value and application potential, and improving yield

Inactive Publication Date: 2016-04-20
西藏天虹科技股份有限责任公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the production of GDL by chemical synthesis involves the application of organic solvents, which inevitably brings residues of organic solvents, and there are problems in product safety.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Yarrowia lipolytica and method for producing gamma-decalactone by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The method of the present invention comprises the following steps:

[0037] 1. Fermentation strain: Yarrowia Lipolytica (Yarrowia Lipolytica) C85 screened by nitrosoguanidine (NTG) mutagenesis # .

[0038] Nitrosoguanidine (NTG) mutagenesis: Centrifuge the seed medium in the logarithmic growth phase of the medium (6000r / min5min), wash with 20mL phosphate buffer each time, centrifuge twice, combine, draw 20ml of bacterial suspension into a In the small Erlenmeyer flask with glass beads wrapped in a black bag, add 0.6ml NTG (preparation method: add 2.5mg NTG, distilled water to 10ml, final concentration is 0.25mg / ml). The colonies with better growth were selected and subjected to shake flask fermentation experiments, and were repeatedly screened by NTG treatment to obtain C85 # , after fermentation, the lactone output can reach more than 8.7g / L.

[0039] 2. Medium:

[0040] (1) Slant medium: wort slant (recipe: malt extract powder 90-120g / L, yeast powder 5-10g / L, agar...

Embodiment 2

[0051] Take the primary seeds in a 500mL Erlenmeyer flask, fill with 150-200mL seed culture medium, inoculum size 10%-15%, culture on a shaker at 25°C, 175r / min for 48h, initial pH 6.0, to obtain secondary seeds.

[0052] Inoculation of large-volume fermentation culture in a 50L fermenter: transfer the secondary seeds into a 50L fermenter (with 30L sterilized fermentation medium inside) according to the inoculum amount of 2%, and use 5% castor methanolic acid methyl ester respectively At 64h, 76h, 92h, and 102h, feed was fed in batches, adding 3% glucose and 2% methyl ricinoleate in a mass-volume ratio to the fermentation medium. The fermentation temperature was adjusted to 27° C. and the fermentation time was 288 hours. The measured concentration of γ-decalactone was 87.53%, the yield was 14.52 g / L, and the conversion rate was 49.34%.

[0053] Among them, the seed medium contains: glucose 15g / L, yeast extract 2.5g / L, peptone 3.5g / L, magnesium sulfate 2.4g / L, potassium dihydro...

Embodiment 3

[0056] Take the primary seeds in a 500mL Erlenmeyer flask, fill with 150-200mL seed culture medium, inoculum size 10%-15%, culture on a shaker at 25°C, 175r / min for 48h, initial pH 6.0, to obtain secondary seeds.

[0057] Inoculation of large-volume fermentation culture in a 50L fermenter: transfer the secondary seeds into a 50L fermenter (with 30L sterilized fermentation medium inside) according to the inoculum amount of 2%, and use 5% castor methanolic acid methyl ester respectively At 64h, 76h, 92h, and 102h, feed was fed in batches, adding 4% glucose and 7% methyl ricinoleate in a mass-volume ratio to the fermentation medium. The fermentation temperature was adjusted to 27° C. and the fermentation time was 288 hours. The measured concentration of γ-decalactone was 85.1%, the yield was 14.9 g / L, and the conversion rate was 49.2%. Among them, the seed medium contains: glucose 18g / L, yeast extract 7.5g / L, peptone 7.5g / L, magnesium sulfate 5g / L, potassium dihydrogen phosphate ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Yarrowia lipolytica C85#. The Yarrowia lipolytica C85# is collected by General Microorganism Center of China Committee for Culture Collection of Microorganisms; the collection number is CGMCC No.11736, and the collection time is November 25th, 2015. The invention also discloses a method for producing gamma-decalactone by using the Yarrowia lipolytica. The method optimizes the high-yield strain fermentation technique, determines the fermentation technological parameters, and enhances the yield of the gamma-decalactone.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, relates to a Yarrowia lipolytica, and also relates to a method for preparing gamma-decalactone by fermentation of the Yarrowia lipolytica. Background technique [0002] γ-Decalactone (γ-Decalactone, GDL) is a lactone fragrance substance, it is a ten-carbon compound containing a five-membered lactone ring, the molecular formula is C 10 h 18 o 2 , the molecular weight is 170.25. GDL is a colorless liquid with coconut, peach, citrus, coconut aroma, boiling point 153°C / 2000Pa or l14-116°C / 66.7Pa, refractive index (nD21.5) 1.4610, slightly soluble in water, easily soluble in organic solvents. It is widely used in the preparation of flavors such as cream, peach, citrus and coconut, and is one of the most demanded products in spices. GDL naturally exists in peaches, strawberries, coconuts, mangoes, apricots, beer, and rum. It has vinegar, fruity, creamy, peach, and coconut aromas. It also...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/16C12P17/04C12R1/645
CPCC12P17/04C12N1/145C12R2001/645Y02P20/54
Inventor 张春颖
Owner 西藏天虹科技股份有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com