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Cordyceps militaris glucan and preparation method and application thereof

A technology of dextran and pupae, which is applied in the field of biologically active substances and their preparation, can solve the problems that the exact structure and preparation method have not been reported.

Active Publication Date: 2016-04-20
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Since 2013, researchers at home and abroad have reported the lipid-lowering effect of Cordyceps militaris extract alone or in combination, but the active ingredients with lipid-lowering function in polysaccharides of Cordyceps militaris fruiting bodies and their exact structures and preparation methods have not been reported.

Method used

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  • Cordyceps militaris glucan and preparation method and application thereof
  • Cordyceps militaris glucan and preparation method and application thereof
  • Cordyceps militaris glucan and preparation method and application thereof

Examples

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Embodiment 1

[0028] When extracting and preparing Cordyceps militaris fruiting body glucan TY517 of the present invention and verifying its lipid-lowering activity, the following steps were taken:

[0029] 1) Preparation of crude polysaccharides from fruiting bodies of Cordyceps militaris: collecting fruiting bodies of Cordyceps militaris, drying in the shade, pulverizing with a plant grinder, and passing through a 100-mesh sieve. Add 10 times the amount of water to the pulverized fruiting body of Cordyceps militaris, stir evenly, and place in an ultra-low temperature refrigerator to freeze at -80°C for 12 hours. Quickly put into 80 ℃ water bath to thaw, water bath for 2 hours. Then 500W ultrasonic treatment for 30 minutes, and the solid matter obtained by filtration is the broken Cordyceps militaris fruiting body. Add 95% ethanol to the fruiting body of Cordyceps militaris after breaking the wall and reflux for 2 hours, the ratio of solid to liquid is 1:5 (W / V), extract the defatted and ...

Embodiment 2

[0048] When extracting and preparing Cordyceps militaris fruiting body glucan TY517 of the present invention and verifying its lipid-lowering activity, the following steps were taken:

[0049] 1) Preparation of crude polysaccharides from fruit bodies of Cordyceps militaris: collect fruit bodies of Cordyceps militaris, dry in the shade, pulverize with a plant crusher, and pass through a 200-mesh sieve. Add 8 times the amount of water to the pulverized fruiting body of Cordyceps militaris, stir evenly, and place in an ultra-low temperature refrigerator to freeze at -40°C for 24 hours. Thaw quickly in a 90°C water bath for 1 hour. Then 200W ultrasonic treatment for 1 hour, the solid matter obtained by filtration is the fruiting body of Cordyceps militaris. Add 95% ethanol to the fruiting body of Cordyceps militaris after breaking the wall and reflux for 3 hours, the ratio of solid to liquid is 1:4 (W / V), extract the defatted and soluble molecules, and filter to obtain solid matt...

Embodiment 3

[0067] The following steps are taken to extract and prepare Cordyceps militaris fruiting body glucan TY517 of the present invention:

[0068] 1) Preparation of crude polysaccharides from fruiting bodies of Cordyceps militaris: collecting fruiting bodies of Cordyceps militaris, drying in the shade, pulverizing with a plant grinder, and passing through a 150-mesh sieve. Add 9 times the amount of water to the pulverized Cordyceps militaris fruiting bodies, stir evenly, and place in an ultra-low temperature refrigerator to freeze at -40°C for 18 hours. Quickly put into 85 ℃ water bath to thaw, water bath for 1.5 hours. Then 350W ultrasonic treatment for 45 minutes, filtered to obtain solid matter as broken Cordyceps militaris fruiting bodies. Add 95% ethanol to the fruiting body of Cordyceps militaris after breaking the wall, reflux for 2.5 hours, the ratio of solid to liquid is 1:4.5 (W / V), extract defatted and soluble molecules, and filter to obtain solid matter. Add distilled...

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Abstract

The invention relates to a Cordyceps militaris fruiting body glucan and a preparation method and application thereof. The preparation method provided by the invention mainly comprises the following steps: baking and crushing Cordyceps militaris fruiting bodies, carrying out ethanol degreasing, carrying out hot-water ultrasonic extraction, and subjecting an extract to concentration, alcohol precipitation, deproteinization, column chromatography, dialysis and freeze drying in succession, thereby obtaining the Cordyceps militaris fruiting body glucan TY517. The Cordyceps militaris polysaccharide provided by the invention has a lipid-lowering effect, and the Cordyceps militaris polysaccharide with the concentration of 25mg / kg can be used for remarkably lowering the cholesterol, triglyceride and low-density lipoprotein cholesterol level of high-fat-induced laboratory animal models and increasing the high-density lipoprotein cholesterol level.

Description

technical field [0001] The invention relates to a bioactive substance and its preparation method and application, in particular to a glucan extracted from the fruit body of Cordyceps militaris and its preparation method and application. Background technique [0002] Cordyceps militaris (Cordyceps militaris L.Link), also known as Cordyceps militaris and Cordyceps militaris, belongs to the same fungus as Cordyceps sinensis. It not only has similar biological activities to Cordyceps sinensis, but also is easy to cultivate artificially, and has become a potential substitute for Cordyceps sinensis. [0003] Polysaccharides are extremely abundant in nature, and have significant biological activities such as immune regulation, anti-tumor, hypoglycemic, and lipid-lowering, which have attracted great attention from the medical community at home and abroad, and have become a research and application hotspot in recent years. Cordyceps militaris polysaccharide is one of the biologically...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K31/716A61P3/06
CPCA61K31/716C08B37/0009
Inventor 郭守东
Owner TAISHAN MEDICAL UNIV
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