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Method for separation and purification of crocin and crocetion from gardenia yellow pigment

A technology of gardenia yellow pigment and crocetin, which is applied in the field of separation and purification of crocetin and crocetin, and can solve the problems of difficulty in realizing large-scale industrial production, complicated operation, and low purification efficiency

Active Publication Date: 2016-04-20
WUHAN GREEN FOOD BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for separating crocin and crocetin from gardenia yellow pigment, which generally has complex operations, low purification efficiency, and difficulty in realizing industrialized large-scale production. Method for separating and purifying crocetin and crocetin in

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]A method for separating and purifying crocin and crocin from gardenia yellow pigment, comprising the following steps:

[0038] (1) rough separation of crocin in gardenia yellow pigment;

[0039] Selecting the gardenia yellow pigment with a color value of 180A as a raw material, diluting it into an aqueous gardenia yellow solution with a color value of 5A, adding citric acid to adjust pH to 5.5, on the D-151 type resin column through activation treatment, and controlling the flow rate of the outflow valve 3.5ml / mim, collect the effluent liquid a; after the resin column is saturated and adsorbed, add pure water 5BV to rinse the resin column;

[0040] The resin column was decomposed with an aqueous ethanol solution with a mass fraction of 70%, and the outflow rate of the desorption solution was controlled to be 2.0ml / min; the desorption solution was collected in three stages, and the concentration of crocin in the desorption solution at the initial stage of desorption was l...

Embodiment 2

[0049] A method for separating and purifying crocin and crocin from gardenia yellow pigment, comprising the following steps:

[0050] (1) rough separation of crocin in gardenia yellow pigment;

[0051] Select the gardenia yellow pigment that color value is 170A as raw material, it is diluted into the gardenia yellow aqueous solution that color value is 5A, add citric acid and adjust pH to 5, go through the D-151 type resin column of activation treatment, control outflow valve flow rate 3.0ml / mim, collect the effluent liquid a; after the resin column is saturated and adsorbed, add pure water 4BV to rinse the resin column;

[0052] The resin column was decomposed with an 80% ethanol aqueous solution, and the outflow rate of the desorption solution was controlled to be 1.5ml / min; the desorption solution was collected in three sections. 0.6-1.0A, collect this section of liquid b; the concentration of crocin in the analysis solution in the middle of the analysis is the highest, an...

Embodiment 3

[0060] A method for separating and purifying crocin and crocin from gardenia yellow pigment, comprising the following steps:

[0061] (1) rough separation of crocin in gardenia yellow pigment;

[0062] Selecting the gardenia yellow pigment with a color value of 200A as a raw material, diluting it into a gardenia yellow aqueous solution with a color value of 5A, adding citric acid to adjust pH to 6, on the D-151 type resin column through activation treatment, and controlling the flow rate of the outflow valve is 4.0ml / mim, collect the effluent liquid a; after the resin column is saturated and adsorbed, add pure water 5BV to rinse the resin column;

[0063] The resin column was decomposed with an aqueous ethanol solution with a mass fraction of 70%, and the outflow rate of the desorption solution was controlled to be 2.5ml / min; the desorption solution was collected in three sections. 0.6-1.0A, collect this section of liquid b; the concentration of crocin in the analysis solutio...

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Abstract

The invention discloses a method for separation and purification of crocin and crocetion from gardenia yellow pigment. The method comprises the following steps: gardenia yellow pigment with the color value of 170-200A is taken as the raw material and diluted into a gardenia yellow water solution with the color value of 5A; adding acid to adjust the pH to be 5-6; a D-151 resin column is adopted to extract crocin from the gardenia yellow pigment; a DEAE-cellulose adsorption column is adopted to refine the crude extracted crocin to prepare crocin powder with the purity of 79-82%, wherein the yield of crocin is 36.8-40%; after adding ethanol into a residual liquid of the crude extracted crocin and the refined crocin, adding ethyl acetate for extraction so as to prepare crocetion with the purity of 69.5-75%, wherein the yield of crocetion is 20-26%. The method provided by the invention starts from the raw material of gardenia yellow pigment, is simple in operation process, high in separation and purification efficiency and large in operation volume, and can realize industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of deep processing of gardenia yellow pigment, in particular to a method for separating and purifying crocin and crocin from gardenia yellow pigment. Background technique [0002] Gardenia yellow pigment is a kind of natural carotene series pigment extracted from gardenia fruit, and its main components contain crocin and crocin. Crocin is the product of the esterification reaction of crocin and gentiobiose. It has good pharmacological effects, anti-oxidation, anti-cancer activity, good health care effect and high safety. It is used in medicine, food, chemical industry. and other fields have a wide range of uses. Crocetin and crocetin are separated from natural gardenia yellow pigment to realize large-scale production and have good application prospects and market potential. [0003] The Chinese patent application number is 201110417356.1, and the title is "a method for preparing crocin and crocin". The dri...

Claims

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Application Information

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IPC IPC(8): C07H13/06C07H1/08C07C51/42C07C57/13
CPCC07C51/42C07H1/08C07H13/06C07C57/13
Inventor 梅元碧王力张伟
Owner WUHAN GREEN FOOD BIOLOGICAL ENG
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