Duck-derived material droplet type digital PCR (Polymerase Chain Reaction) absolute quantitative detection kit

Abstract

The invention relates to a duck-derived material droplet type digital PCR (Polymerase Chain Reaction) absolute quantitative detection kit which mainly comprises droplet digital PCR detection reagents and a plurality of droplet generator cards. The droplet type digital PCR detection reagents include different reagents which are packaged by an outer package and are labeled with names, batch number, production date and period of validity. The kit has the advantages of high sensitivity, accuracy, precision, repeatability and capability of achieving quantitative detection without standard curves, effectively detects counterfeit products, is a rapid, sensitive and accurate duck-derived material detection kit, and provides an effective way to detect duck-derived materials.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a microdroplet digital PCR absolute quantitative detection kit for duck-derived components and a detection method thereof. Background technique [0002] Duck-derived materials are DNA fragments of duck species. [0003] Many of the beef, mutton and meat products sold in the market are mixed with duck meat and sold as beef and mutton. In order to make up for the lack of mutton flavor, some even add additives such as mutton powder and mutton extract to whitewash their adulteration. Serious adulteration and fraudulent behaviors have appeared on the Internet, such as "selling duck meat with a sheep's head", adding mutton flavor to duck meat to make mutton to deceive consumers. This is not only a violation of relevant regulations, but also a kind of commercial fraud. [0004] Meat adulteration and adulteration are important issues in the food industry. In view of the cur...

AI Technical Summary

Problems solved by technology

In recent years, fluorescent PCR technology has been widely used in detection fields such as adulteration identification. It uses PCR technology to efficiently amplify DNA and combines high specificity and sensitivity of probe technology to overcome the shortcomings of tissue identification and ordinary PCR methods. However, the detection limit of fluorescent PCR technology is still very low, and it is impossible to detect a small amount of adulterated samples, so the concept of digital PCR was proposed

[0008] The concept of digital PCR was adopted and published by Bert Vogelstein as early as 1999, and its original intention was to detect a small amount of mutant cells from a large number of normal somatic cells in clinical samples (such as urine, lymph, plasma, feces, etc.) , but because the consumables that could be used to dilute samples at that time were only 384-well plates, it could not reflect the core concept of digital PCR very well - "terminal dilution" (terminal dilution, also called partition in some literature)

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