Microbial growth promoter specially used for ecological planting of wild rice stems and preparation method thereof
A growth promoter and ecological planting technology, applied in the field of microorganisms, can solve the problems of low yield of water jasmine, deterioration of soil and soil quality, and compaction.
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[0030] The invention provides a method for preparing a microbial growth promoter specially used for the ecological cultivation of Zizania zizania, which is characterized in that it comprises:
[0031] 1) Preliminary screening out Pseudomonas, Azotobacter syringae, Bacillus subtilis, Methylomonas and Streptomyces from Zizania japonica planting soil, and further screening to obtain strains with fast growth rates;
[0032] 2) Mixing and culturing the strains obtained in step 1) in a liquid medium to obtain a mixed bacterial liquid;
[0033] 3) performing solid expansion culture on the mixed bacterial solution obtained in step 2) in the solid expansion medium to obtain the initial fermentation product;
[0034] 4) drying the initial fermentation product obtained in step 3), adding a matrix carrier, sieving, and packaging to obtain the microbial growth promoter.
[0035] In one embodiment of the present invention, the specific steps of the preliminary screening are: soaking the Zi...
Embodiment 1
[0054] A preparation method of a microbial growth promoter specially used for the ecological cultivation of Zizania zizania, specifically:
[0055] Step 1: Breeding of Fermentation Strains
[0056] a. Bacterial strain screening: choose well-growing planting soil, add 10 times the weight of the soil to the sterile water and soak for 24 hours, use photosynthetic bacteria medium to screen out Rhodopseudomonas in the leaching solution, and use nitrogen-fixing medium to screen the original Brown Azotobacter, use beef extract peptone agar medium to screen Bacillus subtilis and Methylomonas, use potato sucrose agar medium to screen Streptomyces;
[0057] b. Shake flask count screening: the above strains that were screened were diluted to 10% with 0.9% normal saline. -5 The final bacterial suspension was cultured upside down on a nutrient agar plate at 35°C for 48 hours, and the colony with a fast growth rate was selected for a shake flask experiment to observe the growth rate of the...
Embodiment 2
[0074] A method for preparing a microbial growth promoter specially used for the ecological cultivation of Zizania zizania, specifically:
[0075] Step 1: Breeding of Fermentation Strains
[0076] a. Bacteria screening: select well-growing planting soil, add 10 times the weight of the soil to the sterile water and soak for 26 hours, use photosynthetic bacteria medium to screen out Rhodopseudomonas in the leaching solution, and use nitrogen-fixing medium to screen the original Brown Azotobacter, use beef extract peptone agar medium to screen Bacillus subtilis and Methylomonas, use potato sucrose agar medium to screen Streptomyces;
[0077] b. Shake flask counting and screening: Dilute the above strains with a concentration of 0.9% normal saline to 10-7 and place the bacterial suspension on a nutrient agar plate at 35°C for 48 hours, and select colonies with fast growth Conduct shake flask experiments, observe the growth rate of the strains, and screen the strains with a quanti...
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