Preparation method of high-molecular-weight silver carp skin collagen peptide
A high-molecular-weight, skin-collagen technology, applied in the field of protein peptide preparation in biotechnology, can solve problems such as high cost, non-centralized distribution, and complicated process, and achieve the effect of low cost and high utilization rate of raw materials
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Embodiment 1
[0029] A method for preparing high molecular weight silver carp skin collagen peptide, the steps are as follows:
[0030] (1) Weigh 1000Kg of fresh silver carp skin, rinse to remove foreign matter, soak in 0.2% sodium carbonate solution 4 times the weight of the fish skin for 8 hours, rinse until nearly neutral, then soak in 0.1% hydrochloric acid solution 5 times the weight of the fish skin 3h, rinse to near neutral;
[0031] (2) Put the washed fish skin into the reaction tank, add 2000Kg of pure water, start stirring, adjust the pH of the mixture to 4.5 with dilute hydrochloric acid, heat up to about 42°C by passing steam to the jacket of the reaction tank, and wait until the temperature and pH of the mixture are constant Add 2Kg of pepsin, maintain the initial pH and temperature and stir for 12 hours;
[0032] (3) Concentrate the enzymolysis solution with ultra-low temperature vacuum concentration equipment, the concentration temperature is 40°C, and transfer it to the rea...
Embodiment 2
[0037] A method for preparing high molecular weight silver carp skin collagen peptide, the steps are as follows:
[0038] (1) Weigh 2000Kg of fresh silver carp skin, wash to remove foreign matter, soak in 0.3% sodium hydroxide solution 6 times the weight of the fish skin for 6 hours, rinse until nearly neutral, and then use 0.2% citric acid 5 times the weight of the fish skin Soak in the solution for 6 hours, rinse until nearly neutral;
[0039] (2) Put the washed fish skin into the reaction tank, add 3000Kg of pure water, start stirring, adjust the pH of the mixture to 4.4 with citric acid, heat up to about 45°C by passing steam to the jacket of the reaction tank, and wait until the temperature and pH of the mixture are constant Add 3Kg of pepsin, maintain the initial pH and temperature and stir for 15 hours;
[0040] (3) Concentrate the enzymolysis solution with ultra-low temperature vacuum concentration equipment, the concentration temperature is 45°C, and transfer it to t...
Embodiment 3
[0045] A method for preparing high molecular weight silver carp skin collagen peptide, the steps are as follows:
[0046] (1) Weigh 2500Kg of fresh silver carp skin, rinse to remove foreign matter, soak in 0.4% calcium hydroxide solution 5 times the weight of the fish skin for 10 hours, rinse until nearly neutral, and then use 0.1% nitric acid solution 4 times the weight of the fish skin Soak for 4 hours, rinse until nearly neutral;
[0047] (2) Put the washed fish skin into the reaction tank, add 4000Kg of pure water, start stirring, adjust the pH of the mixture to 3.5 with dilute hydrochloric acid, heat up to about 41°C by passing steam to the jacket of the reaction tank, and wait until the temperature and pH of the mixture are constant Add 2.5Kg of pepsin, maintain the initial pH and temperature and stir for 18 hours;
[0048] (3) Concentrate the enzymolysis solution with ultra-low temperature vacuum concentration equipment, the concentration temperature is 44°C, and trans...
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