Streptomyces lavendulae S3-5 and application thereof to preventing and treating corn gray leaf spots

A technology for Streptomyces lilacinus and Botrytis cinerea, which is applied in the field of microorganisms, can solve the problems of difficulty in eradication, environmental pollution of chemical pesticides, and large limitations, and achieves the effects of simple use method, simple preparation method and high control effect.

Active Publication Date: 2016-03-23
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problems of large limitations, difficulty in eradication, and serious environmental pollution caused by chemical pesticides in the prior art by using physical and chemical methods to prevent and treat gray spot disease of corn, and explores a new source of biological pesticides. The antibacterial effect isolated from the plant soil is the best, and it can be used for the efficient and industrialized production of actinomycetes for the prevention and treatment of corn gray spot. Streptomyces lavendulae ) S3-5

Method used

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  • Streptomyces lavendulae S3-5 and application thereof to preventing and treating corn gray leaf spots
  • Streptomyces lavendulae S3-5 and application thereof to preventing and treating corn gray leaf spots
  • Streptomyces lavendulae S3-5 and application thereof to preventing and treating corn gray leaf spots

Examples

Experimental program
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Effect test

Embodiment 1

[0056] Screening and identification of Streptomyces lilacinus griseus S3-5

[0057] Through the antibacterial test in the dish, 8 strains with inhibitory effect on Cercospora zeae were obtained from the preliminary screening, of which 2 strains were actinomycetes and 6 strains were bacteria. One of the actinomycetes numbered S3-5 has the most obvious antibacterial effect, and the diameter of the inhibition zone is 15 mm, which is the largest among the eight strains. At the same time, the inhibition zone is completely transparent, indicating that within the scope of its metabolite penetration, The hyphal growth of pathogenic bacteria can be completely inhibited, and the strain S3-5 has good antibacterial stability in passage. Through the analysis of the morphological characteristics, cultural characteristics, physiological and biochemical characteristics, and 16SrDNA homology sequence of the strain S3-5, it was determined that the strain S3-5 was Streptomyces lilacinus. And it...

Embodiment 2

[0059] In this example, the fermented liquid of Streptomyces lavender gray S3-5 was prepared, and the fermented liquid was used to prevent and treat gray spot of corn.

[0060] The preparation method of the fermented liquid of Streptomyces lilacinus griseus S3-5 is as follows: After cultivating Streptomyces lilacinus griseus S3-5 on the oatmeal agar medium for 7 days, wash the spores with a small amount of sterile water to make S3-5 spore suspension , inoculate the spore suspension into the PDB liquid medium, the inoculation amount of the spore suspension is 10% of the volume of the PDB liquid medium 28°C, 80r min -1 Cultivate on a shaker for 3 days to obtain the fermentation liquid, store it in a refrigerator at 4°C for later use, and measure the concentration of spores in the fermentation liquid to be 10 6 CFU / mL.

Embodiment 3

[0062] The difference between this embodiment and Example 2 is that: when preparing the Streptomyces lilacaceae S3-5 fermentation broth, the culture time of Streptomyces lilacaceae S3-5 in this embodiment on the oatmeal agar medium was 10 days, and inoculated to After the PDB liquid culture medium, 25 ℃, 180rmin-1 shaker culture 10d, the inoculation amount of spore suspension is 30% of the volume of PDB liquid culture medium, and the spore concentration in the present embodiment fermented liquid is 10 8 CFU / mL.

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Abstract

The invention provides streptomyces lavendulae S3-5 and application thereof to preventing and treating corn gray leaf spots. The preservation number of the streptomyces lavendulae S3-5 is CGMCCNo.11236, the bacterial strain, fermentation liquid of the bacterial strain, antibacterial matter of the bacterial strain, and seed processing agents prepared through the fermentation liquid of the bacterial strain are used for preventing and treating corn gray leaf spots caused by cercospora zeae-maydis (C. zeae-maydis), the preventive effect is high, industrialized production can be achieved, the preparation technology is simple, the using method is simple, and streptomyces lavendulae S3-5 is free of toxin and pollution and environmentally friendly.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a streptomyces lilacinus griseus ( Streptomyces lavendulae ) S3-5 and its application in the control of corn gray spot. Background technique [0002] Gray spot of corn is caused by Cercospora zeae ( C. zeae-maydis) A disease caused by corn is one of the important diseases of corn, which is seriously harmful, widely distributed and difficult to control. Gray spot disease occurs quickly and is difficult to control, and once the disease occurs, it often causes grain failure in the entire planting area, which has brought great harm to my country's corn economy. [0003] At present, physical and chemical methods are mainly used for the prevention and treatment of gray spot disease. For example, through the comprehensive use of disease-resistant varieties, strengthening cultivation management, and combining control agents and other technologies to prevent and control corn gray spot...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/56
Inventor 孙群李玺洋莫婕黄渊余洋冯甦
Owner SICHUAN UNIV
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