New application of ethyl gallate to osteosarcoma treatment

A technology of ethyl gallate and osteosarcoma, which is applied in the field of pharmaceutical application of ethyl gallate, and can solve problems such as unreported research

Inactive Publication Date: 2016-03-23
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although the role of ethyl gallate has been discovered by researchers one after another, its research on the treatment of osteosarcoma, especially U2-OS osteosarcoma, has not been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: During the culturing process of the revived U2-OS osteosarcoma cell line, the cell medium was changed as needed, and after the cell adherent growth area reached 80% of the bottom of the cell bottle, the culture medium was poured out, and the culture medium was added to The sampler draws 2mL PBS into the culture bottle, closes the lid, shakes it several times, pours off the PBS, and repeats this operation once. Use a pipette to draw 1 mL of trypsin into the culture bottle, screw the cap tightly, and place the culture bottle flat so that the trypsin can infiltrate the culture surface. Observed under an inverted microscope, if the cells become a single round shape, it is necessary to terminate the trypsin digestion. After wiping with alcohol cotton, put it into the ultra-clean workbench, pipette 1mL of fresh medium into the culture bottle, use a pipette gun to suck the liquid in the culture bottle and blow the cell culture surface of the culture bottle, and the ...

Embodiment 2

[0027] Example 2: For the passaged cells, pour out the culture medium, suck 2mL PBS into the culture bottle with a sampler, close the lid, shake it several times, pour out the PBS, and repeat this operation once. Use a pipette to draw 1 mL of trypsin into the culture bottle, screw the cap tightly, and place the culture bottle flat so that the trypsin can infiltrate the culture surface. Observed under an inverted microscope, if the cells become a single round shape, it is necessary to terminate the trypsin digestion. After wiping with alcohol cotton, put it into the ultra-clean workbench, pipette 1mL of fresh medium into the culture bottle, use a pipette gun to suck the liquid in the culture bottle and blow the cell culture surface of the culture bottle, and the liquid becomes turbid. Dilute the above-mentioned digested cells with fresh medium, and blow to disperse, absorb a little cell suspension, and drop it on the edge of the cover slip, so that the suspension fills the spac...

Embodiment 3

[0028] Example 3: For the subcultured cells, discard the culture medium, suck 2mL PBS into the culture bottle with a sampler, close the lid, shake it several times, pour out the PBS, and repeat this operation once. Use a pipette to draw 1 mL of trypsin into the culture bottle, screw the cap tightly, and place the culture bottle flat so that the trypsin can infiltrate the culture surface. Observed under an inverted microscope, if the cells become a single round shape, it is necessary to terminate the trypsin digestion. After wiping with alcohol cotton, put it into the ultra-clean workbench, pipette 1mL of fresh medium into the culture bottle, use a pipette gun to suck the liquid in the culture bottle and blow the cell culture surface of the culture bottle, and the liquid becomes turbid. Dilute the above-mentioned digested cells with fresh medium, and blow to disperse, absorb a little cell suspension, and drop it on the edge of the cover slip, so that the suspension fills the sp...

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PUM

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Abstract

Functions of ethyl gallate are found by researchers one by one, but researches in the aspect of osteosarcoma treatment, particularly U2-OS type osteosarcomas, are not reported. The invention discloses new application of ethyl gallate to osteosarcoma treatment, and particularly relates to application of ethyl gallate to treatment of osteosarcomas, especially the U2-OS type osteosarcomas. Ethyl gallate has inhibition functions of different degrees on the osteosarcomas under high dosage, intermediate dosage and low dosage. As time goes by, the treatment effect of high-concentration ethyl gallate is better than 5-fluorouracil with the concentration the same as that of ethyl gallate in the market, and it is possible that ethyl gallate replaces 5-fluorouracil to treat the osteosarcomas.

Description

technical field [0001] The invention relates to a medicine application of ethyl gallate, especially the medicine used in treating U2-OS type osteosarcoma. Background technique [0002] Osteosarcoma (Osteosarcoma) is a malignant tumor originating from the bone, and its incidence rate ranks first among primary bone malignant tumors. 75% of osteosarcoma patients are teenagers, and male patients are 1.5 times higher than female patients. The degree of malignancy is very high, and early lung metastasis is prone to occur. The annual survival rate is only 5-20%, which brings great mental harm to the patients and their families, and also brings a heavy economic burden to the society and even the country. Limb-salvage surgery combined with high-dose chemotherapy has gradually become the first choice for osteosarcoma, but 20-40% of patients still die of metastasis. [0003] In the previous research, we isolated ethyl gallate from Limonium bicolor, which is also known as ethyl 3,4,5-...

Claims

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Application Information

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IPC IPC(8): A61K31/235A61P35/00
CPCA61K31/235
Inventor 马丽滕杰晖李维林任冰如陈剑吕寒
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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