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Rapid propagation method for Gymnadenia conopsea (L.) R. Br.

A bergamot, rapid technology, applied in the field of rapid propagation of bergamot, can solve the problems of low germination rate, unable to grow strong seedlings and other problems, and achieve the effect of improving germination rate, low cost and convenient operation

Inactive Publication Date: 2016-03-23
ZHEJIANG TOP MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This research is also the first non-symbiotic germination research on bergamot seeds, the seed germination rate can reach 20%, which is already a great breakthrough, but the germination rate is still very low, and the protocorm obtained after germination is further induced and differentiated treatment, the highest differentiation rate is only about 7%, and the buds obtained in the later stage of differentiation cannot survive strong seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A rapid propagation method of bergamot ginseng, characterized in that the steps are as follows:

[0042] seed sprouting:

[0043] (1) Preprocessing:

[0044] Take moderately mature seeds of Bergamot ginseng, yellow seeds that are not cracked and easy to peel off from the placenta, rinse with running water for 30 minutes, then add to the pretreatment solution, place in a constant temperature ultrasonic generator (power: 25KHz), and treat at a constant temperature of 28°C for 3 minutes ;

[0045] The formula of the pretreatment liquid is: malic acid 1g / L, laccase 0.8g / L (enzyme activity is 5000u / g), glycine 2g / L, and the rest is pure water;

[0046] (2) Germination medium culture:

[0047] After pretreatment, use neutral detergent to clean, then rinse with distilled water for 5 times, transfer it to a sterile bottle under aseptic conditions; rinse with sterile water for 3 times, rinse with 75% alcohol Disinfect for 20 seconds, rinse with sterile water for 5 times, the...

Embodiment 2

[0070] A rapid propagation method of bergamot ginseng, characterized in that the steps are as follows:

[0071] seed sprouting:

[0072] (1) Preprocessing:

[0073] Take moderately mature seeds of Bergamot ginseng, yellow seeds that are not cracked and easy to peel off from the placenta, rinse with running water for 30 minutes, then add to the pretreatment solution, place in a constant temperature ultrasonic generator (power: 25KHz), and treat at a constant temperature of 28°C for 3 minutes ;

[0074] The formula of the pretreatment liquid is: malic acid 1g / L, laccase 0.8g / L (enzyme activity is 5000u / g), glycine 2g / L, and the rest is pure water;

[0075] (2) Germination medium culture:

[0076] After pretreatment, use neutral detergent to clean, then rinse with distilled water for 5 times, transfer it to a sterile bottle under aseptic conditions; rinse with sterile water for 3 times, rinse with 75% alcohol Disinfect for 20 seconds, rinse with sterile water for 5 times, the...

Embodiment 3

[0099] A rapid propagation method of bergamot ginseng, characterized in that the steps are as follows:

[0100] seed sprouting:

[0101] (1) Preprocessing:

[0102] Take moderately mature seeds of Bergamot ginseng, yellow seeds that are not cracked and easy to peel off from the placenta, rinse with running water for 30 minutes, then add to the pretreatment solution, place in a constant temperature ultrasonic generator (power: 25KHz), and treat at a constant temperature of 28°C for 3 minutes ;

[0103] The formula of the pretreatment liquid is: malic acid 1g / L, laccase 0.8g / L (enzyme activity is 5000u / g), glycine 2g / L, and the rest is pure water;

[0104] (2) Germination medium culture:

[0105] After pretreatment, use neutral detergent to clean, then rinse with distilled water for 5 times, transfer it to a sterile bottle under aseptic conditions; rinse with sterile water for 3 times, rinse with 75% alcohol Disinfect for 20 seconds, rinse with sterile water for 5 times, the...

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PUM

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Abstract

The invention discloses a rapid propagation method for Gymnadenia conopsea (L.) R. Br.. According to the method, various physical and biological means are utilized, cost is low, operation is convenient, and the germination rate of seeds and the differentiation rate of protocorms can be greatly increased; besides, strong seedling culture can be performed, and by transferring seedlings into a greenhouse after seedling hardening, the final survival rate is high.

Description

technical field [0001] The invention relates to a rapid propagation method of Bergamot ginseng, which belongs to the field of biology. Background technique [0002] Orchidaceae Ginseng is a perennial herbaceous plant, there are 10 species in the world, and 5 species in my country, including Southwest Ginseng, Ginseng, Short-range Ginseng, Emei Ginseng and Angular-growth Ginseng. Bergamot ginseng has been included in many Tibetan medicine classics because of its considerable medicinal value. [0003] The seeds of orchids are extremely small (each seed is 1-2mm long and 0.1-0.2mm in diameter), and because the embryo is not fully developed and lacks endosperm to supply nutrients, it needs to live symbiotically with the fungi in the habitat under natural conditions. Germination, the natural germination rate is extremely low. Orchids can be divided into aerial orchids and terrestrial orchids according to their growth environment. In order to adapt to the ecological environment ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 谢再法姚凤鸣郭锋燕
Owner ZHEJIANG TOP MEDICINE
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