Seedling-stage root rot resistance identification method for vigna unguiculata

Abstract

The invention belongs to the field of disease resistance detection of horticultural crops and relates to a seedling-stage root rot resistance identification method for vigna unguiculata. The method comprises the steps of carrying out germination hastening on vigna unguiculata seeds, carrying out sowing and seedling raising, carrying out special strain inoculating so as to carry out screening, preparing a vigna unguiculata root rot bacterium spore suspension, carrying out pathogenic bacterium infestation, carrying out onset examination and the like. Root systems of young seedlings are soaked and infested by adopting a bacterial solution so as to subject the vigna unguiculata young seedlings to artificial root rot bacterium infection; and the vigna unguiculata seeds are cultured in seedling raising matrixes until each young seedling has two leaves and one heart, and then, the young seedlings are drawn out for infestation, so that the growth vigor and size of the vigna unguiculata young seedlings are easily controlled, the vigna unguiculata young seedlings are easily drawn out, and the consistency and reliability of identification results are guaranteed. Special indicator strains are obtained through screening, the infesting effect is better by adopting appropriate time of infestation and appropriate root rot bacterium spore suspension concentration, the bacterium inoculating is uniform, and the onset is rapid. According to the method, by only injuring roots of different plants of consistent growth vigor through directly and naturally rubbing the roots and then infesting the roots with the indicator strains, the root rot resistance of a large amount of vigna unguiculata breeding materials can be identified simply, conveniently, rapidly and reliably, so that the efficiency of variety resistance identification is increased.

Description

technical field [0001] The invention belongs to the technical field of identification of disease resistance of horticultural crops, in particular to a seedling identification method for cowpea root rot resistance, and relates to the technical field of early rapid identification of resistant materials in crop disease resistance breeding. Background technique [0002] Cowpea (scientific name Vignaunguiculata) is commonly known as carob, ginger bean, belt bean, and hanging bean. It belongs to the annual plant of the legume family and is mainly produced in tropical, subtropical and temperate regions. Cowpea has a long history of cultivation in my country, and the planting area is wide. Except for Qinghai and Tibet, it is planted in all provinces and municipalities of the country. Generally, spring and summer cultivation is the main method, followed by autumn cultivation. In recent years, with the growth of the planting area, coupled with the fact that cowpea grows in a hot and ...

AI Technical Summary

Problems solved by technology

However, this identification method depends on the natural disease conditions. If the environmental conditions are not suitable for the disease or the distribution of pathogenic bacteria is uneven in the disease nursery, it is easy to cause misjudgment of resistance, which takes too long and has strong seasonality.

Images