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Medicated leaven thin-layer chromatography detecting method

A thin-layer chromatography and detection method technology, applied in the field of medicinal material detection, can solve problems such as difficulty in controlling the quality of medicinal materials, lack of identification of active ingredients, moisture, ash, etc.

Inactive Publication Date: 2016-03-16
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Ministerial standards and local standards only have simple descriptions of prescription, preparation method, properties, usage and dosage, etc., lack of active ingredient identification and routine inspection items such as moisture and ash, and it is difficult to control the quality of medicinal materials

Method used

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  • Medicated leaven thin-layer chromatography detecting method
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  • Medicated leaven thin-layer chromatography detecting method

Examples

Experimental program
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Effect test

Embodiment 1

[0037] (1) Preparation of the test solution:

[0038] Take 20 g of the medicinal material powder to be tested (through a 40-mesh sieve), add 5 times the amount (100 mL) of methanol and sonicate for 20 minutes, filter, concentrate the filtrate in a water bath, add methanol to dilute to 5 mL, and use it as the test solution.

[0039] (2) Preparation of reference substance solution:

[0040] Take scopoletin reference substance, and use methanol to make a solution containing 0.1mg per 1mL as the reference substance solution.

[0041] Take 20 g of negative control medicinal material powder (through a 40-mesh sieve), add 5 times the amount (100 mL) of methanol and sonicate for 20 minutes, filter, concentrate the filtrate in a water bath, add methanol to dilute to 5 mL, and use it as the negative control medicinal material solution.

[0042] (3) Thin layer detection

[0043] According to the thin-layer chromatography ("Chinese Pharmacopoeia" 2010 edition 1 appendix appendix VIB thi...

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Abstract

The invention discloses a medicated leaven thin-layer chromatography detecting method which comprises the following steps: (1) test solution preparation: taking herb powder to be detected, adding methanol for extraction, filtering, concentrating, and adding methanol to be the constant volume to be used as the test solution; (2) reference solution preparation: preparing the reference solution from a scopoletin comparison product or preparing the reference solution from the scopoletin comparison product and a negative control herb respectively; (3) thin-layer detection: conducting sample application on the test solution and the reference solution on a thin-layer plate, developing by using petroleum ether-ethyl acetate-formic acid as a developing solvent, inspecting and comparing, wherein the ratio of petroleum ether to ethyl acetate to formic acid is (5-7):(3.5-4.5):(0.05-0.15). According to the medicated leaven thin-layer chromatography detecting method, various components in the medicated leaven can be effectively separated, the stable and reliable index component scopoletin can be found, the quality of the medicated leaven herb can be accurately and reliably monitored, and effective guarantee is provided for clinical effects.

Description

technical field [0001] The invention relates to the field of detection of medicinal materials, in particular to a thin-layer chromatography detection method of Liushenqu. Background technique [0002] Six Divine Comedy, also known as Liuqu and Divine Comedy, was first recorded in "Medicine Properties". 2500g, it has the effect of invigorating the spleen and harmonizing the stomach, promoting digestion and regulating the heart. [0003] Clinically, Liu Shenqu is mainly used for indigestion symptoms such as abdominal fullness, vomiting and dysentery caused by improper diet and stagnation of food accumulation. In the 2010 edition of the "Chinese Pharmacopoeia" alone, there are 74 prescriptions containing Liushenqu, and there are as many as 326 prescriptions containing Liushenqu in the ministerial standard, which is the most widely used traditional fermented koji. species, the market demand is large. [0004] At present, the research on Liushenqu mainly focuses on the content...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 吴珊珊胡昌江李梦琪龚晓猛陈志敏胡麟
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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