Embedded type chromatographic column capable of randomly cutting color tape

Abstract

The invention relates to an embedded type chromatographic column capable of randomly cutting a color tape. A core of the embedded type chromatographic column comprises an external support component and an internal columnar film, wherein the external support component has a function of supporting the internal columnar film; the internal columnar film is used for bearing a fixing phase and is matched with an inner wall of the external support component; and a separating process is performed in the fixing phase in the internal columnar film. The external support component is reasonably designed and is provided with an enough large opening, so that the internal columnar film after being separated together with the color tape can be completely taken out and cut. The embedded type chromatographic column is similar to thin-layer chromatography, but is higher in loading volume, is simple in structure, is low in cost, is more efficient and energy-saving and is suitable for wide popularization in the fields of biology, medicine, chemistry, and the like.

Description

technical field [0001] The invention relates to a chromatographic column, in particular to an embedded chromatographic column which can cut color bands arbitrarily. Background technique [0002] Chromatography, also known as chromatography. It is a separation method based on the mechanism of the distribution equilibrium difference between the components of the mixture between the two phases (stationary phase and mobile phase). A prerequisite for the separation of different components is the difference in affinity of the components to the two phases and the ability to partition unevenly into the two phases. Driven by the mobile phase, due to the difference in the affinity of the components in the mixture to the two phases, they flow through the stationary phase at different speeds and flow out of the stationary phase in a certain order, so that the components in the mixture are separated. . According to different adsorption principles, chromatographic techniques mainly inc...

AI Technical Summary

Problems solved by technology

However, traditional chromatographic columns have some disadvantages: 1) When the compounds have been separated in the stationary phase to form independent color bands, the color bands cannot be taken out, and a lot of time and eluents need to be wasted until the color bands are removed from the bottom of the chromatographic column one by one. flow out, and then waste a lot of time and energy to evaporate the solution of the compound to dryness; 2) due to the small opening, after use, it is inconvenient to take out the waste stationary phase from the opening, and a lot of time will be wasted to deal with these waste stationary phases, It is also inconvenient to clean the chromatography column

However, this patent involves more intermediate columns, and the connection process is more cumbersome, and its ability to separate the ribbon depends on the number of intermediate columns. It must be ensured that the same intermediate column cannot have two ribbons, so that the same ribbon can be easily taken out. ; In addition, strict requirements are placed on the tightness of the connection, otherwise solvents and compounds will leak or permeate from the connection; the chromatography column involved in the above patent needs to clean every part after the separation, especially the connection position, which is time-consuming and laborious

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