Triple PCR primer set capable of simultaneously detecting porcine circovirus II, porcine pseudorabies virus and porcine parvovirus and application thereof

Abstract

The invention discloses a triple PCR primer set capable of simultaneously detecting porcine circovirus II, porcine pseudorabies virus and porcine parvovirus and an application thereof. ORF2 encoding genes with porcine circovirus II characteristics (PCV2), gB (gII) encoding genes with porcine pseudorabies virus (PRV) characteristics and encoding genes for VP2 protein of porcine parvovirus (PPV) are screened out from published documents and serve as amplification primers of three gene targets for PCR detection. Three pairs of primers for amplifying the three gene segments are placed in a PCR system, various parameters such as annealing temperature and specificity are adjusted and optimized, and a triple PCR detection method for directly detecting porcine circovirus II, porcine pseudorabies virus and porcine parvovirus from a sample at a time is established. When the triple PCR detection primers are used for detection, specificity is high, sensitivity is high, and detection efficiency is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a triple PCR primer set for simultaneous detection of porcine circovirus type 2, pseudorabies virus and porcine parvovirus and its application. Background technique [0002] Porcine circovirus Ⅱ (PCV2), porcine parvovirus (PPV) and porcine pseudorabies virus (PRV) are important pathogens that are prevalent in intensive pig farms in my country, causing serious damage to the pig industry. economic loss. Because these three viruses are often mixed infection, it is more difficult to analyze and judge the infection and disease of the group. At present, conventional analysis methods such as virus isolation and serological methods have the defects of long time consumption and low sensitivity, which can no longer meet the needs of actual production and disease prevention. [0003] At present, pig immunosuppression and reproductive disorders caused by porcine circovirus type Ⅱ, porcine parvo...

AI Technical Summary

Problems solved by technology

Traditional methods such as virus isolation and identification are time-consuming and labor-intensive. The requirements for experimental conditions are relatively harsh, and it is difficult to carry out in general laboratories and grassroots units. At the same time, limited by sampling conditions, the isolation rate is often low, and traditional serotype diagnosis The method has many shortcomings such as cumbersome and complicated operation, time-consuming and labor-intensive, low specificity and sensitivity

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