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Screening method for IncRNAs, ADSCs, and induced differentiation method for cartilage cells

A screening method, chondrocyte technology, applied in the direction of bone/connective tissue cells, DNA/RNA fragments, animal cells, etc., can solve the problems of long induction differentiation time and low maturity of chondrocytes, and achieve the effect of high maturity

Active Publication Date: 2016-03-16
SHENZHEN MORECELL BIOMEDICAL TECH DEV CO LTD
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Problems solved by technology

In order to overcome the shortcomings of the existing method of in vitro directional induction into chondrocytes that induces differentiation for a long time and the maturity of the obtained chondrocytes is not high
[0010] Another object of the present invention is to provide a method for inducing differentiation of chondrocytes, so as to overcome the shortcomings of the existing in vitro directional induction and differentiation into chondrocytes that the induction and differentiation time is long and the maturity of the obtained chondrocytes is not high

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  • Screening method for IncRNAs, ADSCs, and induced differentiation method for cartilage cells
  • Screening method for IncRNAs, ADSCs, and induced differentiation method for cartilage cells
  • Screening method for IncRNAs, ADSCs, and induced differentiation method for cartilage cells

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[0029] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] On the one hand, the embodiment of the present invention provides a kind of lncRNAs screening method, comprises the steps:

[0031] Step S01: performing directional induction and differentiation of ADSCs into chondrocytes, and sampling the cells on day X, day Y, and day Z of the directional induction differentiation; wherein, X

[0032] Step S02: using transcriptomics RNA-seq deep sequencing technology to perform lncRNAs sequencing on the cells and mature chondrocytes on day X, day Y, and day Z of the directional induced differentiation, respectively;

[0033] Step S03: Usi...

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Abstract

The invention provides a screening method for IncRNAs which are in differential expression during chondrogenic differentiation of ADSCs, ADSCs with high cartilage differentiation capacity, and an induced differentiation method for cartilage cells. The screening method for IncRNAs comprises the following steps: gradient induced differentiation of ADSCs chondroblasts, IncRNAs sequencing of ADSCs, and analysis of IncRNAs sequencing results. The IncRNAs included in the ADSCs comprise at least one selected from the group consisting of H19, Scube3 and NONRATG00281. The ADSCs with high cartilage differentiation capacity are modified by the IncRNAs in differential expression.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a lncRNAs screening method, lncRNAs contained in differentially expressed ADSCs, ADSCs with high cartilage differentiation ability, and a method for inducing differentiation of chondrocytes. Background technique [0002] Injuries and lesions of articular cartilage are common clinical diseases that can occur in any age and gender. Because articular cartilage lacks vascular system, lymphatic system and innervation, and does not contain progenitor cells, and chondrocytes are surrounded by dense cell matrix, the self-repair ability of the tissue is very poor after injury, and once the injury occurs, it will cause joint swelling and pain , to accelerate the progression of osteoarthritis. [0003] At present, clinical methods for repairing articular cartilage damage mainly include articular surface reconstruction methods such as cartilage debridement plastic surgery, subchondra...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113C12N5/0775C12N5/077
CPCC12N5/0655C12N2506/1384C12Q1/6809C12Q2535/122
Inventor 不公告发明人
Owner SHENZHEN MORECELL BIOMEDICAL TECH DEV CO LTD
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