Fluorescence detection instrument and system based on micro-fluidic chip

Abstract

The invention provides a fluorescence detection instrument and system based on a micro-fluidic chip. The instrument comprises an instrument frame, a light source, a micro-fluidic chip supporting structure, a fluorescence collection module, a light path fixing module and a fluorescence detection module, wherein the light source, the micro-fluidic chip supporting structure, the fluorescence collection module, the light path fixing module and the fluorescence detection module are installed on the instrument frame, the light source is used for stimulating a fluorescent substance on the chip to emit fluorescent light, the micro-fluidic chip supporting structure is used for supporting and moving the chip, and the fluorescence collection module is used for filtering, collecting and focusing a fluorescence signal on the micro-fluidic chip onto the fluorescence detection module; the light path fixing module is used for fixing the position of the fluorescence collection module and for light path adjustment, and the fluorescence detection module is used for converting a received optical signal into an analog electrical signal and then converting the analog electrical signal into a digital electrical signal. The instrument is connected with a computer to form a system. The instrument can meet the requirement for fluorescence detection on the micro-fluidic chip. The instrument is high in resolution ratio and sensitivity, simple and compact in structure, small in size, and convenient to carry and use.

Description

technical field [0001] The invention relates to the technical field of microfluidic chip analysis, in particular to a microfluidic chip-based fluorescence detection instrument and system. Background technique [0002] Microfluidic analysis method is a newly developed technology in recent years. This method is mainly based on analytical chemistry, combined with the achievements of biochemistry, molecular biology, physical chemistry, immunology and other related disciplines. Nano-liter to pico-liter volume fluid can be used to analyze and detect the substances on the side. It has the advantages of small volume, large specific surface area, short reaction time, fast analysis speed, less reagent and sample consumption, and simultaneous detection of multiple samples and multiple indicators. The discovery of disease markers and early diagnosis of diseases in research have important practical value. [0003] Conventional diagnostic analysis methods require a relatively long analys...

AI Technical Summary

Problems solved by technology

[0006] However, the current microfluidic chip fluorescence detection devices for immunoassays cannot take into account the volume, resolution and sensitivity, so they cannot meet the requirements of the corresponding instruments for the rapid development of microfluidic chip technology.

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