Lactobacillus plantarum specific culture medium and application thereof

Abstract

The invention discloses a lactobacillus plantarum specific culture medium. The culture medium is convenient to prepare, is low in cost, and contains substances of lincomycin and a carbon source, wherein after cooperative use of lincomycin and the carbon source, the culture medium can be used for culturing lactobacillus plantarums and counting specificity in fermented milk, lactobacillus beverages or bacterial powder. The invention also provides an application of using the lactobacillus plantarum specific culture medium which is used for counting viable lactobacillus plantarums by culturing strains in the culture medium and then performing plate counting on cultured strains; the counting method is simple, is high in specificity, and relative to a PCR counting method, is lower in requirement on operators and lower in cost. The lactobacillus plantarum specific culture medium disclosed by the invention is suitable for preparing a culture medium capable of performing specificity counting on viable lactobacillus plantarums.

Description

technical field [0001] The invention belongs to the field of microbial detection, and relates to a culture medium and a method for counting viable bacteria on bacterial strains, in particular to a specific culture medium for Lactobacillus plantarum and its application. Background technique [0002] Lactobacillus plantarum is a type of Lactobacillus, often found in fermented vegetables and fruit juices, most of which are isolated from plants, so it is named Lactobacillus plantarum. According to my country's "List of Strains Available for Food", Lactobacillus plantarum is one of the probiotic strains that can be used for food. It can well tolerate the gastric acid and bile of the human body and play a role in regulating the balance of intestinal flora. [0003] Studies have shown that Lactobacillus plantarum has beneficial health functions such as enhancing immunity, lowering cholesterol, preventing cardiovascular diseases, and regulating intestinal flora. Therefore, Lactobac...

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Problems solved by technology

[0004] The detection and counting methods of Lactobacillus plantarum are not stipulated in the national standard of our country. At present, the identification or quantitative detection methods of Lactobacillus plantarum are mainly PCR or fluorescent quantitative PCR method of molecular biology, such as the application number 201210594264. Quantitative detection method of Lactobacillus and its detection component box and application” Chinese invention patent application discloses a method for quantitative detection of Lactobacillus plantarum, which is a PCR detection technology, specifically extracting the total RNA of the sample to be tested and reverse transcription For cDNA, specific primers are used for fluorescent quantitative PCR, and the number of Lactobacillus plantarum is determined by comparing the Ct value of the sample to be tested with the standard curve of the quantitative external standard. Although this method produces results quickly, the disadvantage is that this method requires precise Instruments, expensive components and demanding operators

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