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Persea americana oil enzymolysis extraction method

An extraction method and avocado oil technology, applied in the field of bioengineering, can solve the problems of high cost, long cycle, poor oil quality, etc., and achieve the effects of improving oil yield, significant effect, and reducing emissions

Active Publication Date: 2016-02-24
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pressing method is the traditional extraction method of avocado oil, and the oil yield can reach 85%, but the production process has many steps, long cycle, poor quality of oil, high impurity content, high acid value, poor color and luster, which is dark green
Boiling centrifugal separation method has low acid value, good color and high oil yield as high as 80-90%, but the method of colloidal grinding and refining is very serious emulsification, and the demulsification method of adding salt and boiling will produce a bitter taste Substances, the bitter substance will enter into the oil, it is difficult to remove, and the centrifuged waste residue also contains this bitter substance, it is difficult to be used as raw materials for feed, resulting in a large amount of wastewater and waste residues. Subsequent processing problems
Supercritical CO 2 The extraction method has high oil yield, good oil quality, no waste water, and the residue can be reused directly, but the investment is large and the cost is high, and there is no practical value at present

Method used

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  • Persea americana oil enzymolysis extraction method
  • Persea americana oil enzymolysis extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Put 1 kg of fresh avocado pulp (the oil content of the pulp is 18.28%) into a beater, add water and beat, and the resulting pulp passes through an 80-mesh sieve; heat the pulp to 80°C for 5 minutes to inactivate the enzyme activity; The concentration of the live pulp is adjusted so that the ratio of fresh pulp to water is 1:4, and pectinase is added to account for 1.5‰ of the mass of fresh pulp, and the enzymatic hydrolysis is carried out at 45°C for 12 hours, and 60.8g of oil is separated, and then the avocado oil is counted The extraction rate (see Table 1 for details).

Embodiment 2

[0029] Put 1 kg of fresh avocado pulp (the oil content of the pulp is 18.28%) into a beater, add water and beat, and the resulting pulp passes through an 80-mesh sieve; heat the pulp to 80°C for 5 minutes to inactivate the enzyme activity; The concentration of the live pulp is adjusted so that the ratio of fresh pulp to water is 1:4, cellulase is added to account for 3.0‰ of the mass of fresh pulp, and the enzymatic hydrolysis is carried out at 55°C for 12 hours, and 112.3g of oil is separated, and then the avocado oil is counted The extraction rate (see Table 1 for details).

Embodiment 3

[0031] Put 1 kg of fresh avocado pulp (the oil content of the pulp is 18.28%) into a beater, add water and beat, and the resulting pulp passes through an 80-mesh sieve; heat the pulp to 80°C for 5 minutes to inactivate the enzyme activity; The concentration of live pulp is adjusted to the ratio of fresh pulp and water of 1:4, adding dextranase to account for 1.5‰ of the mass of fresh pulp, and heat preservation at 55°C for enzymolysis for 12 hours, 121.8g of oil is separated, and then the oil is counted The extraction rate of pear oil (see Table 1 for details).

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to a persea americana oil enzymolysis extraction method which comprises the following steps: pulping, enzyme denaturalixation, pulp concentration adjustment, enzymolysis and oil-water-residue separation. According to the method, in the whole processing process, the temperature of materials is not higher than 80 DEG C, so that bitter materials cannot be generated, and the obtained persea americana oil is less in impurity, low in acid value and good in color and luster. The method adopts the enzymolysis means to damage the cell structure of pulp, oil can be separated out as far as possible, the effect is obvious, the oil extraction rate is greatly improved, and the extraction ratio of persea americana oil can reach 94.26% to the maximum extend.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an enzymolysis extraction method of avocado oil. Background technique [0002] Avocado (Persea Americana Mill), also known as avocado, originates in areas such as Central America and Mexico, belongs to tropical and subtropical famous and precious fruit trees, and is a kind of Lauraceae Avocado. It is cultivated in Hainan, Guangdong, Guangxi, Fujian, Taiwan, Yunnan, Sichuan and Zhejiang in China. Avocado fruit is rich in nutrition, and its pulp contains a large amount of dietary fiber, and the fat content is also high, with an oil content of 8-29%. Avocado oil is a non-drying oil, non-irritating, low acidity, long-term preservation after emulsification, and is widely used as skin moisturizing oil and ointment raw material for high-grade cosmetics, mechanical lubrication and medicine in addition to food. [0003] At present, the extraction methods of avocado oi...

Claims

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Application Information

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IPC IPC(8): C12P7/64
Inventor 刘大胜
Owner GUANGXI UNIV
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