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Application of Astragalus polysaccharide in bone marrow mesenchymal stem cells

A technology of bone marrow mesenchyme and astragalus polysaccharide, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve problems such as outstanding safety problems

Inactive Publication Date: 2016-02-24
GANSU UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Bone marrow mesenchymal stem cells (BMSCs) have become one of the best stem cells for the cytological treatment of tumors such as lung cancer, but because of their malignant transformation in the tumor environment, their safety issues have become increasingly prominent

Method used

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  • Application of Astragalus polysaccharide in bone marrow mesenchymal stem cells
  • Application of Astragalus polysaccharide in bone marrow mesenchymal stem cells
  • Application of Astragalus polysaccharide in bone marrow mesenchymal stem cells

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Experimental program
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Effect test

Embodiment 1

[0038] 1. Astragalus polysaccharide inhibits the expression of tumor-associated proteins in BMSCs in tumor cell co-culture system and its effect on improving genetic stability-biological factors

[0039] 1 material

[0040] 1.1 Source of cells

[0041] BMSCs were purchased from Cyagen Biosciences Inc. (CatalogNumber: HUXMA-01001; RegistrationNumber: 08795844465781); A549 cell line was purchased from Shanghai Cell Institute, Chinese Academy of Sciences (CatalogNumber: TCHu150); astragalus polysaccharide (APS) was purchased from Yuanye Biotechnology Co., Ltd., Cat. No. YY91332.

[0042] 2 methods

[0043]2.1 Cell proliferation and toxicity test (MTT colorimetric method) to screen the effective parts and effective concentration of Astragalus

[0044] 2.2.1 Preparation of cell samples Take untreated BMSCs and A549 cells in the logarithmic growth phase, respectively use DMEM / F12 and DMEM medium containing 10% fetal bovine serum to prepare a concentration of about 5×10 4 cells / m...

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Abstract

The invention provides application of Astragalus polysaccharide in bone marrow mesenchymal stem cells. The application refers to effect, on protecting proliferative and genetic stability of the bone marrow mesenchymal stem cells, of Astragalus polysaccharide. Researches show that Astragalus polysaccharide has protective effect on damage to the bone marrow mesenchymal stem cells caused by various factors, and the Astragalus polysaccharide can serve as drug for preventing malignant transformation of damage.

Description

technical field [0001] The invention relates to the application of astragalus polysaccharide in bone marrow mesenchymal stem cells. Background technique [0002] Bone marrow mesenchymal stem cells (BMSCs) have become one of the best stem cells for the cytological treatment of tumors such as lung cancer, but because of their malignant transformation in the tumor environment, their safety issues have become increasingly prominent. Research on the mechanism of malignant transformation of BMSCs in the lung cancer microenvironment and screening of drugs that can prevent its malignant transformation have received increasing attention. The principle and technology of epigenetics has become one of the important ways to explore the mechanism of tumorigenesis and to screen the effective parts of traditional Chinese medicines for preventing and treating tumors. [0003] Astragalus has the effects of invigorating qi and raising yang, benefiting health and solidifying the surface, diure...

Claims

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Application Information

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IPC IPC(8): C12N5/0775A61K31/715A61P35/00
Inventor 刘永琦何建新舍雅丽张志明雍文兴张月梅
Owner GANSU UNIV OF CHINESE MEDICINE
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