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Microfluidic chip for reconstructing bone cell microenvironment and its application method

A microfluidic chip, bone cell technology, applied in the method of supporting/fixing microorganisms, bone/connective tissue cells, tissue cells/virus culture devices, etc., can solve the problem of difficult to truly reflect the physiological state of bone cells biological characteristics question

Active Publication Date: 2017-08-25
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the same fluid stress will be formed on the cell body and cell synapse surface of bone cells in the traditional fluid chamber, which is difficult to reflect the biological characteristics of bone cells in the physiological state objectively.

Method used

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  • Microfluidic chip for reconstructing bone cell microenvironment and its application method
  • Microfluidic chip for reconstructing bone cell microenvironment and its application method
  • Microfluidic chip for reconstructing bone cell microenvironment and its application method

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Embodiment Construction

[0028] Before introducing the present invention, the principle of chip design is explained first. Please refer to figure 1 , there is a thiol molecule (EG-terminated thiols, referred to as EG6) whose functional group is polyethanol long chain (PEG), the molecular formula is (HS(CH 2 ) 11 (OCH 2 CH 2 ) 6 OH). This thiol molecule can react with metallic gold (Au) to form a gold-sulfur bond on one side of the sulfur atom (S). The other end of the thiol can resist protein adsorption because of the presence of PEG, thereby preventing cells from adsorbing on the Au surface.

[0029] The present invention combines microfluidic chip technology with bone cell culture technology, and uses calcium alginate polymer to cover the bone cell body and expose the cell synapse by limiting the directional growth of cell synapses along nanometer-sized channels. Finally, fluid oscillating flow stimulation was introduced to better simulate the microenvironment of bone cells under physiologica...

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Abstract

The invention provides a micro-fluidic chip used for reconstructing the bone cell microenvironment and a use method thereof. The micro-fluidic chip is characterized in that a two-dimensional grid pattern with gold as a substrate is constructed, the surface of the pattern is subjected to hydrophobic modification through soaking sulfur alcohol, and therefore cells orderly grow along the pattern; meanwhile, bone cell bodies are covered with calcium alginate polymers, and cell synapsis is exposed. In this way, fluid stress is amplified at the synapsis portions, the problem that in a traditional bone cell fluid stress stimulating experiment, same fluid stress is formed on the surfaces of the cell bodies and the surfaces of the cell synapsis is solved, and the biological characteristics of the bone cells in the physiological status can be more truly reflected.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a microfluidic chip for rebuilding the microenvironment of bone cells and its application method. Background technique [0002] Osteoporosis can cause limb function loss complications such as fractures, which seriously affects the independent living ability of patients and reduces the quality of life. In China, 63 million people suffer from osteoporosis in varying degrees. In the metabolism of bone tissue, osteocytes can realize the remodeling of bone tissue by regulating the functions of osteoblasts and osteoclasts, which is considered to be a potential breakthrough point that can essentially solve osteoporosis. Therefore, the study of osteocytes has great potential. very important meaning. [0003] As a new research tool, microfluidic technology can control, manipulate and detect fluids at the microscopic scale. Because its characteristic size is comparable to that of cells, it h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/00C12N5/071C12N11/14C12N11/10
CPCB01L3/5027C12M23/16C12M25/14C12N5/0654C12N11/10C12N11/14
Inventor 陈健范蓓媛卫超卫元晨陈德勇王军波
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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