A high and medium pressure chromatographic column with shrinkage and smooth connection at both ends

Abstract

The invention relates to a high- and medium-pressure chromatographic column with shrinkage and smooth connection at both ends, which comprises a column tube, a column head and a column tail. Based on the specially designed column head, the present invention can more effectively complete the distribution of the sample at the column head; and based on the design of the shrinking column tail, it can produce a certain sample concentration effect while completing the separation. Its overall effect is reflected in: the overall separation length is increased, and the actual separation length is equivalent to the sieve plate at the column head to the sieve plate at the end of the column; the utilization rate of the stationary phase is greatly improved, and the stationary phase at the column head and the end of the column can be more efficient. Make full use of it; effectively reduce the production cost of the column tube, because no specially designed distribution plate is used, and a small sieve plate is used instead of a large sieve plate with the same inner diameter as the column tube. Therefore, while increasing the separation effect and increasing the production efficiency per unit time, the production cost of the column system can be effectively reduced.

Description

【Technical field】 [0001] The invention relates to the technical field of chromatographic columns, in particular to a high- and medium-pressure chromatographic column with shrinkage and smooth connection at both ends. 【Background technique】 [0002] In 1966, H.M.Stahr et al. [H.M.Stahr, R.M.Ikeda, E.T.Oakley, B.M.Carter.Anal.Chem., 1966, 38: 19741 of the Philip Marris Research Center in the United States made a low-pressure dimensional liquid chromatography column for the first time. They used polyethylene Plastic is used as the column material, and it is confirmed that the column efficiency of the tapered column is significantly better than that of the first-order cylindrical column. In 1990, Moerker Theophile of Switzerland invented a tapered column [Switzerland Patent CH675212, 1990] with the inner diameter of the inlet smaller than the inner diameter of the outlet. When the sample flows in the column, it will gradually diffuse to the tube wall. In such a tapered column w...

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Problems solved by technology

[0003] Chinese Patent Publication No. CN1204402C relates to a conical HPLC preparative column. By adjusting the flow rate, a flow pattern close to that of a plug can be obtained; due to the large inner diameter of the opening, the amount of sample loading increases, but at the same time it also brings The problem of difficult distribution of samples

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