Method for producing artemisinic acid through fermentation
A technology of artemisinic acid and fermentation tank, which is applied in the field of industrial microorganisms, can solve the problems of production strain toxicity, etc., and achieve the effects of low production cost, reduced production process, and simple and easy method
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Embodiment 1
[0041] Put 1ml of the cryopreserved seed solution into a 750ml shake flask containing 100ml of culture medium, culture at 30°C for 24h, put it into a fermenter containing 7L of fermentation medium, and culture at 30°C, stirring at 200rpm, with an air flow rate of 420L / h. Increase the stirring speed and air flow according to the change of dissolved oxygen, and control the dissolved oxygen above 20%. After 14 hours of fermentation, start to add glucose and ethanol, the concentration of glucose is 30%, add at a rate of 7ml per hour (about 0.1% of the volume of the fermentation broth); the concentration of ethanol is 70%, add at a rate of 14ml per hour (about 0.1% of the fermentation broth volume) 0.2% of the liquid volume) was added at a rate.
[0042] When cultivating for 24 hours, start to add n-hexane, the supplement rate is 70ml / (1%) day, continue to supplement until 140 hours, put the tank, and centrifuge to obtain an organic phase of about 300ml. HPLC detection shows that t...
Embodiment 2
[0044] Put 1ml of the cryopreserved seed solution into a 750ml shake flask containing 100ml of culture medium, culture at 30°C for 24h, put it into a fermenter containing 7L of fermentation medium, and culture at 30°C, stirring at 200rpm, with an air flow rate of 420L / h. Increase the stirring speed and air flow according to the change of dissolved oxygen, and control the dissolved oxygen above 20%. After 16 hours of fermentation, start to add glucose and ethanol. The concentration of glucose is 50%, and it is added at a rate of 28ml per hour (about 0.4% of the volume of the fermentation broth); the concentration of ethanol is 95%, and it is added at a rate of 35ml per hour (about 0.5% of the liquid volume) was added at a rate.
[0045] When culturing for 24 hours, start to add n-hexane, the supplement rate is 210ml / (3%) day, continue to supplement until 140 hours, put in the tank, centrifuge to obtain an organic phase of about 950ml, HPLC detection shows that the product conta...
Embodiment 3
[0047] Put 1ml of the cryopreserved seed solution into a 750ml shake flask containing 100ml of culture medium, culture at 30°C for 24h, put it into a fermenter containing 7L of fermentation medium, and culture at 30°C, stirring at 200rpm, with an air flow rate of 420L / h. Increase the stirring speed and air flow according to the change of dissolved oxygen, and control the dissolved oxygen above 20%. After 18 hours of fermentation and cultivation, start to add glucose and ethanol. The concentration of glucose is 60%, and it is added at a rate of 70ml (1%) per hour; the concentration of ethanol is 99.7%, and it is added at a rate of 56ml (0.8%) per hour.
[0048] When cultivating for 48 hours, start to add toluene at a rate of 350ml / (5%) day, continue to add until 140 hours, put in the tank, and centrifuge to obtain an organic phase of about 1300ml. HPLC detection shows that it contains 104g of product, and the average fermentation unit is 14.8g / L.
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