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Method for producing artemisinic acid through fermentation

A technology of artemisinic acid and fermentation tank, which is applied in the field of industrial microorganisms, can solve the problems of production strain toxicity, etc., and achieve the effects of low production cost, reduced production process, and simple and easy method

Active Publication Date: 2016-02-10
CHONGQING QIANTAI BIOLOGICAL MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the fermentation process of artemisinic acid, as the amount of product continues to accumulate, it will cause toxicity to the production strain itself, thereby inhibiting the continued synthesis of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Put 1ml of the cryopreserved seed solution into a 750ml shake flask containing 100ml of culture medium, culture at 30°C for 24h, put it into a fermenter containing 7L of fermentation medium, and culture at 30°C, stirring at 200rpm, with an air flow rate of 420L / h. Increase the stirring speed and air flow according to the change of dissolved oxygen, and control the dissolved oxygen above 20%. After 14 hours of fermentation, start to add glucose and ethanol, the concentration of glucose is 30%, add at a rate of 7ml per hour (about 0.1% of the volume of the fermentation broth); the concentration of ethanol is 70%, add at a rate of 14ml per hour (about 0.1% of the fermentation broth volume) 0.2% of the liquid volume) was added at a rate.

[0042] When cultivating for 24 hours, start to add n-hexane, the supplement rate is 70ml / (1%) day, continue to supplement until 140 hours, put the tank, and centrifuge to obtain an organic phase of about 300ml. HPLC detection shows that t...

Embodiment 2

[0044] Put 1ml of the cryopreserved seed solution into a 750ml shake flask containing 100ml of culture medium, culture at 30°C for 24h, put it into a fermenter containing 7L of fermentation medium, and culture at 30°C, stirring at 200rpm, with an air flow rate of 420L / h. Increase the stirring speed and air flow according to the change of dissolved oxygen, and control the dissolved oxygen above 20%. After 16 hours of fermentation, start to add glucose and ethanol. The concentration of glucose is 50%, and it is added at a rate of 28ml per hour (about 0.4% of the volume of the fermentation broth); the concentration of ethanol is 95%, and it is added at a rate of 35ml per hour (about 0.5% of the liquid volume) was added at a rate.

[0045] When culturing for 24 hours, start to add n-hexane, the supplement rate is 210ml / (3%) day, continue to supplement until 140 hours, put in the tank, centrifuge to obtain an organic phase of about 950ml, HPLC detection shows that the product conta...

Embodiment 3

[0047] Put 1ml of the cryopreserved seed solution into a 750ml shake flask containing 100ml of culture medium, culture at 30°C for 24h, put it into a fermenter containing 7L of fermentation medium, and culture at 30°C, stirring at 200rpm, with an air flow rate of 420L / h. Increase the stirring speed and air flow according to the change of dissolved oxygen, and control the dissolved oxygen above 20%. After 18 hours of fermentation and cultivation, start to add glucose and ethanol. The concentration of glucose is 60%, and it is added at a rate of 70ml (1%) per hour; the concentration of ethanol is 99.7%, and it is added at a rate of 56ml (0.8%) per hour.

[0048] When cultivating for 48 hours, start to add toluene at a rate of 350ml / (5%) day, continue to add until 140 hours, put in the tank, and centrifuge to obtain an organic phase of about 1300ml. HPLC detection shows that it contains 104g of product, and the average fermentation unit is 14.8g / L.

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PUM

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Abstract

The invention provides a method for producing artemisinic acid through fermentation. The method includes: adding a small amount of an organic solvent into a fermentation broth; transferring a product into an organic phase from the fermentation broth. The objectives of prolonging biosynthesis time, improving biosynthesis efficiency, increasing yield and simplifying extraction process are achieved. By the technical scheme, production procedures are reduced, and production efficiency is improved; the method is simple and easy to implement and suitable for industrialized production.

Description

technical field [0001] The invention relates to the technical field of industrial microorganisms, in particular to a method for fermenting and producing artemisinic acid. Background technique [0002] Malaria is a disease that threatens the health of 300 to 500 million people around the world. In 2010, a total of 200 million people were infected with malaria worldwide, and at least 655,000 people died. [0003] Artemisinin (artemisine) is a peroxy group-containing sesquiterpene lactone antimalarial drug extracted from the traditional Chinese medicine Artemisia annua. It is the first internationally recognized natural drug discovered in China. Derivatives such as dihydroartemisinin, artemether, artesunate, etc. Artemisinin drugs have low toxicity and strong antimalarial properties, and have been approved by the WTO as the first choice for the treatment of cerebral malaria and falciparum malaria worldwide. Antimalarial drugs such as artemisinin are widely used in Africa and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/18C12R1/865
Inventor 刘省伟别一郭明袁建栋
Owner CHONGQING QIANTAI BIOLOGICAL MEDICINE
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