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Method for extracting vitamin K2 from vitamin K2 embedding medium and method for detecting vitamin K2

A technology for vitamins and embedding agents, which is applied in the field of analytical chemistry and can solve the problems of difficult determination of vitamin K2 content, unstable vitamin K2, inability to determine vitamin K2 and the like

Inactive Publication Date: 2016-02-03
BY HEALTH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The microcapsule embedding membrane is a polymer material, and vitamin K2 is a fat-soluble vitamin. Organic solvents must be used to extract vitamin K2, but organic solvents cannot dissolve the polymer microcapsule embedding membrane, and vitamin K2 is unstable, resulting in the content of vitamin K2 The determination is very difficult; therefore, none of the methods reported in the literature can measure vitamin K2 embedded in microcapsules

Method used

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  • Method for extracting vitamin K2 from vitamin K2 embedding medium and method for detecting vitamin K2
  • Method for extracting vitamin K2 from vitamin K2 embedding medium and method for detecting vitamin K2
  • Method for extracting vitamin K2 from vitamin K2 embedding medium and method for detecting vitamin K2

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Embodiment 1

[0071] Embodiment 1 chromatographic conditions

[0072] Mobile phase: isopropanol: methanol: 0.1% acetic acid solution = (500:475:25)

[0073] Flow rate: 1.0ml / min

[0074] Wavelength: 270nm

[0075] Column temperature: 40°C Injection volume: 20μL

[0076] Chromatographic column: AgilentSB-C18, 4.6×250mm, 5μm

Embodiment 2

[0077] Embodiment 2 chromatographic conditions

[0078] Mobile phase: isopropanol: methanol: 0.1% acetic acid solution = (400:450:30)

[0079] Flow rate: 0.8ml / min Wavelength: 270nm

[0080] Column temperature: 35°C Injection volume: 20μL

[0081] Chromatographic column: AgilentSB-C18, 4.6×250mm, 5μm

Embodiment 3

[0082] Embodiment 3 chromatographic conditions

[0083] Mobile phase: isopropanol: methanol: 0.1% acetic acid solution = (450:500:15)

[0084] Flow rate: 1.2ml / min Wavelength: 270nm

[0085] Column temperature: 38°C Injection volume: 20μL

[0086] Chromatographic column: AgilentSB-C18, 4.6×250mm, 5μm

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Abstract

The invention relates to the field of analytical chemistry, in particular to a method for extracting vitamin K2 from a vitamin K2 embedding medium and a method for detecting the vitamin K2. According to the methods, the vitamin K2 embedding medium and acid are mixed, then subjected to ultraphonic processing, and then mixed with an organic solvent, the mixture is subjected to ultraphonic processing, and then the mixture is mixed with an organic solvent. The linearity, precision, repeatability, stability, blank, detection limit, quantitation limit and recovery experiments are carried out on the method for measuring the content of the vitamin K2, all the indexes meet the requirement of the GB / T27404-2008 laboratory quality control standard, it proves that the content measuring method is scientific and effective, and the quality control aim on the content of the vitamin K2 in the vitamin K2 compound preparation (embedding medium) can be achieved.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for extracting vitamin K2 from a vitamin K2 embedding agent and a method for detecting vitamin K2. Background technique [0002] Vitamin K2, also known as menaquinone, its structure is composed of a menaquinone mother ring and an isoprene side chain at the C-3 position of the mother ring, according to the number of isoprene units on the side chain According to the difference in number, vitamin K2 is divided into 14 kinds, represented by MK-n, and MK-7 contains 7 isoprene units on the side chain. Vitamin K2 has pharmacological activities such as promoting blood coagulation, preventing and treating osteoporosis, improving arteriosclerosis, preventing and treating liver cancer and leukemia. [0003] Vitamin K2 is unstable when exposed to alkali and light, and is easy to decompose, but resistant to heat and acid. The stability of vitamin K2 in the compound preparation i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
Inventor 苏昭仑叶少文张喜金王德伟
Owner BY HEALTH CO LTD
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