DNA label, PCR primer and application thereof

Abstract

The invention discloses a DNA label, a PCR primer and application thereof. The DNA label is chosen from nucleotides shown as SEQ ID NO:1-95 and can be used for building a nucleic acid sequencing library to accurately differentiate the nucleic acid sequencing library. The DNA label and the PCR primer are utilized to structure a label PCR primer, and deafness gene mutation detection of 95 DNA samples can be realized at most in one time by utilizing the label PCR primer according to a method for determining whether deafness genes of various DNA samples are mutational or not.

Description

technical field [0001] The present invention relates to the technical field of nucleic acid sequencing and typing, in particular, to DNA tags, PCR primers and applications thereof, more specifically, to a set of DNA tags, a set of PCR primers, a method for constructing a nucleic acid sequencing library, and determination of deafness in DNA samples A method for determining whether there is a mutation in a gene, a kit for determining whether a DNA sample has a mutation in a deaf gene, and a system for determining whether a DNA sample has a mutation in a deaf gene. Background technique [0002] Deafness is a common condition that seriously affects quality of life and communication. According to the second national sample survey of disabled people in 2006, there were 82.96 million people with disabilities in my country, including 27.8 million people with hearing disabilities, and 800,000 deaf-mute children under the age of 7, with an annual growth rate of 30,000 newborn deaf chi...

AI Technical Summary

Problems solved by technology

These detection methods have many limitations, such as low detection capacity, low throughput, time-consuming and labor-intensive, expensive equipment and consumables. High-throughput detection of mutation sites, however, the gene chip platform is expensive and requires high quality equipment and personnel, making it difficult to popularize in the vast medical institutions in my country

[0006] And the detection methods described above have low throughput

Applying the above methods is time-consuming, labor-intensive, and costly when performing deafness genetic testing on large-scale samples

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